毕赤酵母水解工艺的研究  

Study on the Hydrolysis Process of Pichia yeast

作　　者：袁稚雯 王士岩 朱小燕 李相前 YUAN Zhiwen;WANG Shiyan;ZHU Xiaoyan;LI Xiangqian(School of Life Sciences and Food Engineering,Huaiyin Institute of Technology,Huaian 223003,China;Provincial Key Construction Laboratory of Probiotics Preparation in Jiangsu,Huaian 223003,China)

机构地区：[1]淮阴工学院生命科学与食品工程学院,江苏淮安223003 [2]江苏省益生制剂重点建设实验室,江苏淮安223003

出　　处：《工业微生物》2023年第5期92-96,共5页Industrial Microbiology

摘　　要：在发酵过程中产生的总体积为30%~40%的毕赤酵母菌体,大部分在发酵后,会直接把酵母菌体排入环境中,不仅浪费资源,还污染环境,因此,实现资源再利用是文章的研究目的。最终结果表明,对毕赤酵母自溶破壁的最佳自溶水解条件为50℃、pH 6.0,作用时间为30 h、4%Na Cl,酵母悬浮液终体积分数为10%。此外,通过对木瓜蛋白酶、中性蛋白酶、碱性蛋白酶和酸性蛋白酶进行研究发现,添加木瓜蛋白酶效果最佳,添加后的酵母水解物氨基氮得率为4.5%,固形物得率为59%,粗蛋白含量为45.38%。During the fermentation process,a total volume of 30%-40%of Pichia pastoris was produced.Most yeast cells were directly discharged into the environment after fermentation,which not only wastes resources but also pollutes the environment.Therefore,achieving resource reuse was the research content.The results showed that the optimal conditions for autolysis and hydrolysis of Pichia pastoris were 50℃,pH 6.0,reaction time of 30 hours,4%NaCl,and the final concentration of yeast suspension was 10%.In addition,through research on papain,neutral protease,alkaline protease,and acidic protease,it was found that adding papain had the best effect.The added yeast hydrolysate had an amino nitrogen yield of 4.5%,a solid yield of 59%,and a crude protein content of 45.38%.

关 键 词：毕赤酵母 酵母水解物 水解工艺 蛋白酶 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]