Development of a novel virus-like particle-based vaccine for preventing tick-borne encephalitis virus infection  

作　　者：Jielin Tang Muqing Fu Chonghui Xu Bao Xue Anqi Zhou Sijie Chen He Zhao Yuan Zhou Jizheng Chen Qi Yang Xinwen Chen 

机构地区：[1]Guangzhou National Laboratory,Guangzhou,510005,China [2]State Key Laboratory of Virology,Wuhan Institute of Virology,Center for Biosafety Mega-Science,Chinese Academy of Sciences,Wuhan,430071,China [3]Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,510530,China [4]GMU-GIBH Joint School of Life Sciences,Guangzhou Medical University,Guangzhou,511436,China [5]State Key Laboratory of Respiratory Disease,Guangzhou Medical University,Guangzhou,511436,China

出　　处：《Virologica Sinica》2023年第5期767-777,共11页中国病毒学（英文版）

基　　金：This work was supported by grants from the National Key Research and Development Program of China(grant number:2018YFA0507201 to X.W.C.);the National Science Foundation of China(grant number:32000111 to Q.Y.);the China Postdoctoral Science Foundation(grant number:2020T130021ZX to Q.Y.and grant number:2020M672580 to Q.Y.).

摘　　要：Tick-borne encephalitis virus(TBEV)is an important tick-borne pathogen that poses as a serious public health concern.The coverage and immunogenicity of the currently available vaccines against TBEV are relatively low;therefore,it is crucial to develop novel and effective vaccines against TBEV.The present study describes a novel strategy for the assembly of virus-like particles(VLPs)by co-expressing the structural(core/prM/E)and non-structural(NS2B/NS3Pro)proteins of TBEV.The efficacy of the VLPs was subsequently evaluated in C57BL/6 mice,and the resultant IgG serum could neutralize both Far-Eastern and European subtypes of TBEV.These findings indicated that the VLP-based vaccine elicited the production of cross-subtype reactive antibodies.The VLPs provided protection to mice lacking the type I interferon receptor(IFNAR^(-/-))against lethal TBEV challenge,with undetectable viral load in brain and intestinal tissues.Furthermore,the group that received the VLP vaccine did not exhibit significant pathological changes and the inflammatory factors were significantly suppressed compared to the control group.Immunization with the VLP vaccine induced the production of multiple-cytokine-producing antiviral CD4+T cells in vivo,including TNF-α^(+),IL-2^(+),and IFN-γ^(+)T cells.Altogether,the findings suggest that noninfectious VLPs can serve as a potentially safe and effective vaccine candidate against diverse subtypes of TBEV.

关 键 词：Tick-borne encephalitis virus(TBEV) Virus-like particle(VLP) IMMUNOGENICITY NEUTRALIZATION VACCINE 

分 类 号：R512.3[医药卫生—内科学]