岩藻多糖对高脂饮食诱导的非酒精性脂肪性肝病的预防作用  

The prevention of high⁃fat diet⁃induced non⁃alcoholic fatty liver disease in mice by Fucoidan

作　　者：刘希鹏 孙娟[1] 张海峰[1] LIU Xi-peng;SUN Juan;ZHANG Hai-feng(Department of Clinical Nutrition,Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine,Shanghai 200011,China)

机构地区：[1]上海交通大学医学院附属第九人民医院临床营养科,上海200011

出　　处：《海南医学院学报》2023年第20期1526-1530,1538,共6页Journal of Hainan Medical University

基　　金：上海市卫生和计划生育委员会科研课题青年项目(20174Y0201)。

摘　　要：目的:研究岩藻多糖对高脂饮食诱导的非酒精性脂肪性肝病的预防作用。方法:将实验小鼠随机分为3组:对照组、高脂饮食组和岩藻多糖干预组。对照组喂食标准饲料,其余两组喂食高脂饲料。对照组和高脂饮食组每天给予生理盐水灌胃,岩藻多糖干预组给予岩藻多糖溶液灌胃,剂量为100 mg∙kg^(-1)∙d^(-1),每天灌胃一次,持续干预12周。最后一次灌胃12 h后,测量各组小鼠的体重和肝脏重量,并计算肝指数。使用生化试剂盒检测各组小鼠肝脏组织中谷丙转氨酶(ALT)、谷草转氨酶(AST)、总胆固醇(TC)和甘油三酯(TG)的含量变化。利用苏木精-伊红染色比较各组小鼠肝组织的病理形态变化。测定各组小鼠肝组织中炎症因子白细胞介素-6、肿瘤坏死因子和氧化应激指标丙二醛的含量,以及谷胱甘肽过氧化物酶和超氧化物歧化酶的活性变化。结果:与对照组相比,接受高脂饲料的小鼠体重和肝指数明显增加(P<0.01)。此外,高脂饮食组小鼠肝脏组织中的TG、TC、AST和ALT含量均较对照组显著增加(P<0.01)。高脂饮食组小鼠的肝脏组织也出现了明显的病理性变化,并伴随着炎症因子表达的增加和氧化应激反应的显著增加。与高脂饮食组小鼠相比,接受岩藻多糖干预的小鼠的肝组织中上述指标均得到显著改善(P<0.01)。结论:岩藻多糖可以抑制高脂饮食引起的肝脏脂质沉积以及肝脏炎症和氧化应激反应。Objective:To study the preventive effect of fucoidan on non-alcoholic fatty liver disease induced by high fat di-et.Methods:The experimental mice were randomly divided into three groups:control group,high-fat diet group and fucoidan in-tervention group.The control group was fed a standard diet,and the other two groups were fed a high-fat diet.The control group and the high-fat diet group were given normal saline intragastric administration every day,and the intervention group was given in-tragastric administration of fucoidan polysaccharide solution at a dose of 100 mg∙kg^(-1)∙d^(-1)once a day for continuous intervention for 12 weeks.After the last intragastric administration for 12 h,the body weight and liver weight of each group of mice were mea-sured,and the liver index was calculated.The contents of alanine aminotransferase(ALT),aspartate aminotransferase(AST),total cholesterol(TC)and triglyceride(TG)in liver tissues of mice in each group were detected by biochemical kit.Hematoxylin-eosin staining(HE)was used to compare the pathological morphological changes of liver tissue in each group.The contents of in-flammatory factor interleukin-6(IL-6),tumor necrosis factor(TNF-α)and oxidative stress index malondialdehyde(MDA),and the activities of glutathione peroxidase(GSH-Px)and superoxide dismutase(SOD)in liver tissues of mice in each group were de-termined.Results:Compared with the control group,the body weight and liver index of mice receiving high fat diet increased sig-nificantly(P<0.01).In addition,the contents of TG,TC,AST and ALT in liver tissue of high-fat diet group were significantly increased compared with that of control group(P<0.01).The liver tissue of mice in the high-fat diet group also showed significant pathological changes,accompanied by increased expression of inflammatory factors and a significant increase in oxidative stress re-sponse.However,compared with the mice in the high-fat diet group,the above indexes were significantly improved in the liver tis-sue of the mice treated with fucoi

关 键 词：岩藻多糖 高脂饮食 非酒精性脂肪肝 炎症反应 氧化应激反应 

分 类 号：R575[医药卫生—消化系统]