Naringenin suppresses NLRP3 inflammasome activation via the mRNA-208a signaling pathway in isoproterenol-induced myocardial infarction  

作　　者：Ayman Eldourghamy Toka Hossam Mohammed Abdalla Hussein Amal Abdel-Aziz Samir A.El-masry 

机构地区：[1]Environmental Biotechnology Department,Genetic Engineering and Biotechnology Research Institute,Sadat University,Egypt [2]Department of Medical Labs,Faculty of Applied Medical Sciences Technology,October 6 University,Egypt [3]Department of Biotechnology,Faculty of Applied Health Sciences Technology,October 6 University,Egypt [4]Molecular Biology Department,Genetic Engineering and Biotechnology Research Institute,Sadat University,Egypt

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2023年第10期443-450,共8页亚太热带生物医学杂志（英文版）

摘　　要：Objective:To investigate the cardioprotective effect of naringenin against isoproterenol(ISO)-induced cardiotoxicity in rats.Methods:Rats were divided into five groups:the normal group,the ISO group(85 mg/kg b.w.);the ISO+naringenin(50 mg/kg b.w.)group,the ISO+naringenin(100 mg/kg b.w.)group and the ISO+propranolol(10 mg/kg b.w.)group.Plasma creatine kinase-MB(CK-MB),cardiac troponin T,lactate dehydrogenase,brain natriuretic peptide(BNP),and IL-10,as well as cardiac transforming growth factor-β1(TGF-β1),vascular endothelial growth factor(VEGF)and malondialdehyde(MDA)were examined.In addition,NLRP3 and mRNA-208a expressions were evaluated by RT-PCR analysis.Histopathological examination was also performed to assess cardiac damages.Results:Naringenin treatment significantly decreased plasma lactate dehydrogenase,CK-MB,cardiac troponin T,BNP,and IL-10,as well as cardiac TGF-β1,VEGF,and MDA while increasing p-Akt and superoxide dismutase in ISO-administered rats.It also reduced NLRP3 and mRNA-208a gene expression levels.Furthermore,naringenin improved ISO-induced cardiac damage.Conclusions:Naringenin attenuates myocardial dysfunction in ISO-treated rats by decreasing oxidative stress and increasing cardiac endogenous antioxidant system,which may be modulated partly by improvement of NLRP3 and mRNA-208a gene expression.

关 键 词：NARINGENIN ISOPROTERENOL Myocardial infarction Antioxidants NLRP3 mRNA-208a 

分 类 号：R285[医药卫生—中药学]