光质对等鞭金藻的生长和生物活性产物合成的影响  

Effect of different light qualities on the growth and bioactive compounds biosynthesis of Isochrysis sp.ISO-FJ

作　　者：钟彩荣 陈钦常 许涵玥 曾子航 徐绍丝 车丹丹 方静平 何勇锦[1,2] ZHONG Cai-rong;CHEN Qin-chang;XU Han-yue;ZENG Zi-hang;XU Shao-si;CHE Dan-dan;FANG Jing-ping;HE Yong-jin(College of Life Sciences,Fujian Normal University;Engineering Research Center of Industrial Microbiology,Ministryof Education,Fujian Normal University,Fuzhou 350117,China)

机构地区：[1]福建师范大学生命科学学院 [2]福建师范大学工业微生物教育部工程研究中心,福州350117

出　　处：《天然产物研究与开发》2023年第10期1775-1781,共7页Natural Product Research and Development

基　　金：国家自然科学基金(41906096);福建师范大学碳中和研究院开放项目(TZH-2022-03);福建省环保科技计划(2023R001)。

摘　　要：本实验探究白光(400~700 nm)、红光(620~650 nm)和绿光(500~580 nm)对等鞭金藻(Isochrysis sp.ISO-FJ)的生长和生物活性产物积累的协同影响,以期摸索适宜的光质同时提高其生物活性产物。实验结果表明,等鞭金藻细胞在白光条件下具有最大的生物量。在生物活性产物合成方面,与白光和红光相比,采用绿光培养的等鞭金藻细胞更有利于岩藻黄素、胞外多糖和多不饱和脂肪酸的联产合成。这些结果可表明,等鞭金藻的生长和活性产物合成是受不同光质的调节,为今后定向调控等鞭金藻生长和活性产物合成提供实践依据和理论指导。In order to explore the appropriate light quality to simultaneously improve multiple bioactive compounds productivities,the synergistic effects of white light(400-700 nm),red light(620-650 nm)and green light(500-580 nm)on the microalgal growth and bioactive compounds biosynthesis of Isochrysis sp.ISO-FJ were investigated.The results showed that Isochrysis sp.ISO-FJ gave the highest biomass in the white light.In term of the bioactive compounds,the green light was more beneficial to the coproduction of fucoxanthin,exopolysaccharides and polyunsaturated fatty acids in microalgal cells,in comparison to the white or red light.These results together suggested that the growth and bioactive compounds biosynthesis of Isochrysis sp.were regulated by light qualities,which could offer practical basis and theoretical guidance for the directed regulation of microalgal growth and bioactive compounds by marine microalgae Isochrysis strains.

关 键 词：等鞭金藻 光质 岩藻黄素 胞外多糖 多不饱和脂肪酸 

分 类 号：Q935[生物学—微生物学]