二氧化碳还原用甲酸脱氢酶的研究进展  

Advances in formate dehydrogenase for carbon dioxide reduction

作　　者：史红玲[1,2] 王瑶 袁书玮 姚伦广 唐存多[1] 薛闯[2] SHI Hongling;WANG Yao;YUAN Shuwei;YAO Lunguang;TANG Cunduo;XUE Chuang(College of Life Science and Agricultural Engineering,Nanyang Normal University,Nanyang 473061,Henan,China;School of Bioengineering,Dalian University of Technology,Dalian 116024,Liaoning,China;School of Chemistry and Chemical Engineering,Henan Normal University,Xinxiang 453007,Henan,China)

机构地区：[1]南阳师范学院生命科学与农业工程学院,河南南阳473061 [2]大连理工大学生物工程学院,辽宁大连116024 [3]河南师范大学化学化工学院,河南新乡453007

出　　处：《精细化工》2023年第10期2089-2097,2206,共10页Fine Chemicals

基　　金：国家自然科学基金项目(31900916);河南省青年人才托举工程项目(2021HYTP036);河南省高校科技创新人才(21HASTIT041);南阳师范学院青年项目(2023QN007)。

摘　　要：CO_(2)的还原和资源化利用是缓解温室效应的重要手段。生物催化剂对反应和底物具有高选择性,因此被用于构建高效的CO_(2)还原系统。其中,甲酸脱氢酶(FDH),特别是某些烟酰胺腺嘌呤二核苷酸(NAD+)依赖型/金属辅因子(W或Mo)的甲酸脱氢酶,能够可逆地将CO_(2)还原成甲酸盐。该文首先介绍了甲酸脱氢酶的特性及分类,其次综述了CO_(2)还原用甲酸脱氢酶的活性位点及催化机制、FDH的分子改造、全细胞生物催化CO_(2)还原为甲酸盐的最新进展,为CO_(2)还原用生物催化剂的研究提供了启示,为以CO_(2)为原料构建可行的CO_(2)还原系统来生产增值的燃料和化学品提供了理论基础。The reduction and resource utilization of CO_(2)are important means to alleviate greenhouse effects.Biocatalysts,with high selectivity for reactions and substrates,are used to build efficient CO_(2)reduction systems.Among them,formate dehydrogenase(FDH),especially thoseβ-nicotinamide adenine dinucleotide(NAD+)-dependent/metal cofactor(W or Mo)FDH are able to reversibly reduce CO_(2)to formate.Herein,the characteristics and classification of formate dehydrogenase were summarized.The active sites and catalytic mechanism of formate dehydrogenase for CO_(2)reduction,molecular modification of the enzyme,and the latest progress in the whole-cell biocatalysis were discussed.The review provides a great enlightenment and guidance for the future research of FDH for CO_(2)reduction,as well as a theoretical basis for the construction of feasible CO_(2)refining systems with CO_(2)as raw materials to produce value-added fuels and chemicals.

关 键 词：甲酸脱氢酶 CO_(2)还原 酶工程 全细胞催化 甲酸盐 

分 类 号：X701[环境科学与工程—环境工程] TQ426.97[化学工程]