Association analysis of BclI with benign lymphoepithelial lesions of the lacrimal gland and glucocorticoids resistance  

作　　者：Xu-Juan Zhang Peng-Xiang Zhao Ming-Shen Ma Hao Wu Rui Liu Hui Wang Meng-Yu Liu Fei Xie Xue-Mei Ma 

机构地区：[1]Faculty of Environment and Life,Beijing University of Technology,Beijing 100124,China [2]Beijing International Science and Technology Cooperation Base of Antivirus Drug,Beijing 100124,China [3]Beijing Tongren Hospital,Capital Medical University,Beijing 100730,China [4]State Key Laboratory of Military Stomatology&National Clinical Research Center for Oral Diseases&Shaanxi International Joint Research Center for Oral Diseases,Department of General Dentistry and Emergency,School of Stomatology,Air Force Medical,Xi’an 710032,Shaanxi Province,China

出　　处：《International Journal of Ophthalmology(English edition)》2023年第11期1734-1745,共12页国际眼科杂志（英文版）

基　　金：Supported by Natural Science Foundation of Beijing(No.7222025);Beijing Hospitals Authority’Ascent Plan(No.DFL20190201);Natural Science Foundation of Beijing Projects(No.81602408).

摘　　要：AIM:To evaluate the relationship between gene polymorphism(BclI,ER22/23EK,N363S)and the occurrence,progression and sensitivity to glucocorticoid of lacrimal gland benign lymphoepithelial lesion(LGBLEL).METHODS:Clinical peripheral blood samples of 52 LGBLEL patients and 10 normal volunteers were collected for DNA extraction and polymerase chain reaction sequencing to analyze single nucleotide polymorphism(SNP)genotypes.The lacrimal tissues of LGBLEL were surgically removed and made into paraffin sections for subsequent hematoxylin-eosin(HE)and Masson staining analysis.The duration of disease and hormone use of LGBLEL patients from diagnosis to surgery were also analyzed.The Meta-analysis follows PRISMA guidelines to conducted a systematic review of human studies investigating the relationship between the NR3C1 BclI polymorphism and glucocorticoids(GCs)sensitivity.RESULTS:There was no association between ER22/23EK or N363S and the occurrence of LGBLEL or GCs sensitivity(P>0.05);BclI GC genotype was closely related to GCs resistance(P=0.03)as is the minor allele C(P=0.0017).The HE staining and Masson staining showed that the GC genotype of BclI remarkably slowed down the disease progression and reduced fibrosis(P<0.05),especially for GCs-dependent patients(P<0.0001).Meta-analysis showed that BclI was not significantly associated with GCs responsiveness.CONCLUSION:The LGBLEL patients who carry the NR3C1 BclI allele C may be more sensitive to GCs and associated with lower fibrosis and slower disease progression.The results may guide the clinical treatment strategy for the LGBLEL patients.

关 键 词：lacrimal gland benign lymphoepithelial lesion Bcll single nucleotide polymorphisms glucocorticoids resistance FIBROSIS 

分 类 号：R739.7[医药卫生—肿瘤]