Therapeutic effect of folic acid combined with decitabine on diabetic mice  

作　　者：Gang Du Yong Yan Jun-Feng Gao Chun-Yan Guo Xiao Shen Xun-Wen Lei 

机构地区：[1]The First Hospital of Lanzhou University,Lanzhou 730000,Gansu Province,China [2]Huining Second People’s Hospital,Baiyin 730700,Gansu Province,China [3]The Fourth People’s Hospital of Tianshui,Tianshui 741000,Gansu Province,China [4]Dingxi People’s Hospital,Dingxi 743000,Gansu Province,China

出　　处：《International Journal of Ophthalmology(English edition)》2023年第11期1766-1772,共7页国际眼科杂志（英文版）

基　　金：Supported by the Natural Science Foundation of Gansu Province(No.21JR7RA361).

摘　　要：AIM:To evaluate the therapeutic effect of folic acid combined with decitabine on diabetic mice.METHODS:The diabetic model of db/db mice were randomly divided into model group,folic acid group,decitabine group,folic acid combined with decitabine group,and C57 mice as normal control group.The density of retinal blood vessels and retinal thickness were detected by fundus photography and optical coherence tomography,respectively.Pathological changes of retina were observed by hematoxylin-eosin(HE)staining.The homocysteine(Hcy)in serum was detected by enzyme linked immunosorbent assay(ELISA).TdT-mediated dUTP nick-end labeling(TUNEL)was used to detect apoptosis in retinal tissue.Evans blue dye was used to detect the permeability of retinal blood vessels.The platelet endothelial cell adhesion molecule-1(CD31)and vascular endothelial growth factor receptor(VEGFR)protein were detected by Western blot.The 3-nitrotyrosine(3-NT)and 4-hydroxynonanine(4-HNE)were detected by immunohistochemistry.RESULTS:The density of retinal blood vessels,retinal thickness,retinal vascular permeability and the proportion of apoptotic cells of retinal tissue in the model group increased significantly than control group(P<0.05).The Hcy in serum and the levels of CD31,VEGFR,3-NT,and 4-HNE in retinal tissue increased significantly in the model group(P<0.01).Folic acid and decitabine both reversed these changes significantly,and the combination of the folic acid and decitabine worked best.CONCLUSION:The combination of folic acid and decitabine has a more significant protective effect on the retina in diabetic mice.

关 键 词：diabetic model folic acid DECITABINE APOPTOSIS MOUSE 

分 类 号：R587.2[医药卫生—内分泌] R774.1[医药卫生—内科学] R-332[医药卫生—临床医学]