Polyphenolic Compounds and Antioxidant Activity of Sea Buckthorn(Hippophae rhamnoides L.)  

作　　者：Ain Raal Linda Rusalepp Tatiana Chiru Nicolae Ciobanu Kelly Talvistu Michelle Shusta Oleh Koshovyi Tõnu Püssa 

机构地区：[1]Institute of Pharmacy,Faculty of Medicine,University of Tartu,Tartu,50411,Estonia [2]Chair of Veterinary Biomedicine and Food Hygiene,Institute of Veterinary Medicine and Animal Sciences,Estonian University of Life Sciences,Tartu,51006,Estonia [3]Faculty of Pharmacy,Nicolae Testemitanu State Medical and Pharmaceutical University,Chisinau,MD-2001,Republic of Moldova [4]Pharmacognosy Department,The National University of Pharmacy,Kharkiv,61002,Ukraine

出　　处：《Phyton-International Journal of Experimental Botany》2023年第11期2965-2979,共15页国际实验植物学杂志(英文)

基　　金：supported by the European Union in the MSCA4Ukraine project ID Number 1232466.

摘　　要：The fruits of the Sea buckthorn(Hippophae rhamnoides L.)are a popular plant food and a valuable health product.Cultivating plants produces a lot of leaves with fine branches as an unnecessary residue,which needs valorization.The aim of the study was to estimate(by HPLC-MS/MS)the qualitative and quantitative content of polyphenolic compounds in different parts of H.rhamnoides(HR),and to determine the level of antioxidant activity of leaves and fruits(by DPPH,ABTS methods and ferrozine test).Up to 19 compounds were identified in different parts of HR.The fruits are rich in flavonoids,including various glycosides of flavonols isorhamnetin,quercetin,and kaempferol.Two isorhamnetin glycosides were not identified in the leaves,while isorhamnetin-3-rhamnosylglactosides and the ellagitannins hippophaenin B,stachyurin and casuarinin were present only in the leaves of the plant.The bark and roots contained considerably more catechins,but minimal flavonols than the fruits and leaves of HR.The total phenolics and flavonols were most abundant in water infusions from leaves of HR(especially quercetin-3-O-glucoside-7-O-rhamnoside),compared to methanolic extracts.No significant differences in the quantitative and qualitative content of the fresh and dried leaves were detected.The highest antioxidant activity by all three methods was detected in the leaves of HR.In addition to the fruits,the leaves may have a perspective as a source of biologically active substances of HR.

关 键 词：BARK FLAVONOLS FRUITS leaves PHENOLS ROOTS 

分 类 号：R28[医药卫生—中药学]