紫苏多糖硒酸改性及理化结构特性分析  

Selenization Modification and Physicochemical and Structural Characterization of Polysaccharides from Perilla frutescens L.Leaves

作　　者：赵亚娜[1,2] 郭江涛 周田田 李孟昊 李会珍[1,2] ZHAO Yana;GUO Jiangtao;ZHOU Tiantian;LI Menghao;LI Huizhen(School of Chemistry and Chemical Engineering,North University of China,Taiyuan 030051,China;Jinzhong Institute of Industrial Technology and Innovation,North University of China,Jinzhong 030600,China;Heze Branch,Qilu University of Technology(Shandong Academy of Sciences),Heze 274000,China)

机构地区：[1]中北大学化学与化工学院,山西太原030051 [2]中北大学晋中产业技术创新研究院,山西晋中030600 [3]齐鲁工业大学(山东省科学院)菏泽分院,山东菏泽274000

出　　处：《食品科学》2023年第20期94-99,共6页Food Science

基　　金：山西省应用基础研究计划项目(20210302124239);山西省高等学校科技创新项目(20200L0287)。

摘　　要：以水提醇沉法提取紫苏多糖(Perilla frutescens polysaccharides,PFPS),经初级分离后,采用HNO_(3)-Na_(2)SeO_(3)法对PFPS进行硒酸改性修饰得到硒化-紫苏多糖(Se-PFPS),并采用傅里叶变换红外光谱(Fourier transform infrared spectroscopy,FTIR)、纳米粒度仪、高效凝胶渗透色谱(high performance gel permeation chromatography,HPGPC)、扫描电子显微镜(scanning electron microscopy,SEM)、X射线衍射(X-ray diffraction,XRD)仪和热重分析仪(thermal gravimetric analyzer,TGA)对2种多糖进行了理化及结构特性分析。原子荧光光谱分析显示硒化-紫苏多糖(Se-PFPS)硒含量可达(1004.33±48.60)μg/g;FTIR分析显示Se-PFPS在620 cm^(-1)处存在Se—O—C特殊吸收峰,表明Na2SeO3与PFPS发生了化学结合,且以亚硒酸酯的形式存在;HPGPC、粒径分析显示化学修饰减小了多糖的颗粒尺寸,但同时增加了多糖分子质量;SEM和XRD分析显示硒酸改性改变了多糖的形态特征和晶体状态,且多以多晶和非晶形式存在;TGA分析显示PFPS比Se-PFPS的热稳定性更强。In this study,polysaccharides(PFPS)were extracted form Perilla frutescens L.and modified by HNO_(3)-Na_(2)SeO_(3) method,and the physicochemical and structural characteristics of PFPS and selenized PFPS(Se-PFPS)was analyzed by Fourier transform infrared(FTIR)spectroscopy,nanoparticle size analyzer,high performance gel permeation chromatography(HPGPC),scanning electron microscopy(SEM),X-ray diffraction(XRD)and thermal gravimetric analyzer(TGA).The selenium content of Se-PFPS was(1004.33±48.60)μg/g as determined by atomic fluorescence spectrometry(AFS).The FTIR spectrum showed a characteristic absorption peak of Se‒O‒C at 620 cm^(-1),indicating that sodium selenite was chemically bound to PFPS and seleniumin in Se-PFPS existed in the form of selenite.HPGPC and particle size analysis showed that selenization modification reduced the particle size,and increased the molecular mass of PFPS.The results of SEM and XRD showed that selenization modification changed the morphology and crystalline state of PFPS,and Se-PFPS mainly existed in polycrystalline and amorphous forms.TGA analysis showed that PFPS had stronger thermal stability than did Se-PFPS.

关 键 词：紫苏 多糖 硒化修饰 硒多糖 结构表征 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]