基于UPLC-MS/MS的毛竹笋不同生长阶段差异代谢物分析  

Analysis of Differential Metabolites of Phyllostachys edulis Shoots at Different Growth Stages by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：郭明阳 贺曰林 潘凯婷 鲍方艳 应叶青[1] GUO Mingyang;HE Yuelin;PAN Kaiting;BAO Fangyan;YING Yeqing(State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300,China)

机构地区：[1]浙江农林大学省部共建亚热带森林培育国家重点实验室,浙江杭州311300

出　　处：《食品科学》2023年第20期283-291,共9页Food Science

基　　金：浙江省与中国林业科学研究院省院合作林业科技项目(2020SY07)。

摘　　要：通过广泛靶向代谢组学结合基础生理指标测定,对毛竹笋破土期(S1-5 cm)、生长期(S2-20 cm)和快速生长期(S3-40 cm)3个时期差异代谢物的动态变化展开研究。生理指标测定结果表明,破土期具有较好的营养风味品质。通过代谢组学共鉴定出765个代谢产物,多元统计分析表明不同时期代谢物成分差异性明显,差异累积代谢物有203个,其中脂质类、氨基酸类、有机酸类、酚酸类以及黄酮类和生物碱类变化较为显著。破土期较好的营养风味品质主要同多数黄酮类、游离脂肪酸、氨基酸以及新绿原酸的富集密切相关。而在竹笋生长期和快速生长期主要包含脂质类、氨基酸衍生物、有机酸类、酚酸类以及褪黑激素,其中仍含有大量的生物活性成分,它们与提高竹笋的环境适应力密切相关。本研究结果可为毛竹笋的营养价值研究以及毛竹资源的开发与利用提供理论依据。The dynamic changes of differential metabolites of Phyllostachys edulis shoots at three stages including ground breaking(S1,5 cm high),growth(S2,20 cm high)and rapid growth(S3,40 cm high)were studied by widely targeted metabolomics combined with basic physiological indices.The results of physiological analyses showed that the nutritional and flavor quality was better at the ground breaking stage than at the other growth stages.A total of 765 metabolites were identified by metabolomics.Multivariate statistical analysis showed that the composition of metabolites varied significantly among growth stages.There were 203 differentially accumulated metabolites identified,among which lipids,amino acids,organic acids,phenolic acids,flavonoids and alkaloids remarkably changed.The nutritional quality at the ground breaking stage could be closely related to the enrichment of most flavonoids,free fatty acids,amino acids and neochlorogenic acid.At the latter two stages,bamboo shoots mainly contained lipids,amino acid derivatives,organic acids,phenolic acids and melatonin as well as a large number of bioactive ingredients,which could be closely related to improving the environmental adaptability of bamboo shoots.The results of this study provide a theoretical basis for understanding the nutritional value of P.edulis shoots and for the exploitation and utilization of bamboo resources.

关 键 词：毛竹笋 不同生长阶段 代谢组学 差异代谢物 营养品质 

分 类 号：S644.2[农业科学—蔬菜学]