重组大肠杆菌产L-阿拉伯糖异构酶的条件优化  

Optimization production of L-arabinose isomerase by recombinant Escherichia coli

作　　者：李俊凯 张艳芳 周金龙 张春枝[1] LI Junkai;ZHANG Yanfang;ZHOU Jinlong;ZHANG Chunzhi(School of Biological Engineering,Dalian Polytechnic University,Dalian 116034,China)

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034

出　　处：《大连工业大学学报》2023年第5期339-343,共5页Journal of Dalian Polytechnic University

基　　金：辽宁省教育厅2021年度科学研究经费项目(LJKZ0514).

摘　　要：L-阿拉伯糖异构酶可以将D-半乳糖催化为D-塔格糖,为提高E.coli BL21(DE3)/pET-30a(+)-araA产L-阿拉伯糖异构酶的能力,分别优化了该基因工程菌发酵基础培养基中的碳源、氮源和诱导时机、诱导温度、诱导剂浓度和诱导时长。结果表明,采用优化后的发酵基础培养基和诱导产酶条件生产的L-阿拉伯糖异构酶的酶活力可达到80.1 U/mL,是优化前的1.8倍。L-阿拉伯糖异构酶与90 g/L的D-半乳糖在55℃的水浴振荡器中反应12 h,D-半乳糖的转化率可达到44.2%。L-arabinose isomerase can catalyze D-galactose to D-tagatose.To improve the ability of E.coli BL21(DE3)/pET-30a(+)-araA to produce L-arabinose isomerase,the carbon and nitrogen sources in the fermentation basal medium of this genetically engineered bacterium were optimized,while the induction timing,induction temperature,induction agent concentration and induction duration were also optimized.The results showed that the enzyme activity of L-arabinose isomerase produced under the optimized fermentation basal medium and induced enzyme production conditions could reach 80.1 U/mL,which was 1.8 times higher than before.L-arabinose isomerase reacted with 90 g/L of D-galactose in a water bath shaker at 55℃for 12 h,and the conversion of D-galactose reached 44.2%.

关 键 词：L-阿拉伯糖异构酶 D-塔格糖 重组大肠杆菌 条件优化 

分 类 号：TQ922.2[轻工技术与工程—发酵工程]