引起李树叶枯病的镰刀菌种类鉴定  

Identification of the species of Fusarium causing plum leaf blight

作　　者：鲁萌萌 马立安[1] 唐利华[2] 陈小林[2] 郭堂勋[2] 黄穗萍[2] 李其利[2] LU Mengmeng;MA Lian;TANG Lihua;CHEN Xiaolin;GUO Tangxun;HUANG Suiping;LI Qili(College of Life Sciences,Yangtze University,Jingzhou 434025,China;Institute of Plant Protection,Guangxi Academy of Agricultural Sciences/Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs/Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests,Nanning 530007,China)

机构地区：[1]长江大学生命科学学院,荆州434025 [2]广西壮族自治区农业科学院植物保护研究所/农业农村部华南果蔬绿色防控重点实验室/广西作物病虫害生物学重点实验室,南宁530007

出　　处：《植物病理学报》2023年第4期713-716,共4页Acta Phytopathologica Sinica

基　　金：广西重点研发计划(桂科AB20159041)。

摘　　要：李(Prunus salicina Lindl.)属于蔷薇科李属落叶植物,栽培历史悠久,我国是李种植面积和年产量最大的国家[1]。近年来,随着李树种植面积的扩大,叶枯病的发生日趋严重[2];发病初期,在叶尖或叶缘形成褪绿的小斑点,并逐渐扩大形成灰褐色不规则病斑,后期叶片出现大面积深褐色病斑,最终导致叶片枯死脱落(图1-A、B)。To identify the pathogen responsible for leaf blight of plum in Meishan and Aba Tibetan and Qiang Autonomous Prefecture in Sichuan Province,six diseased leaf samples were collected and 11 Fusarium-like isolates were obtained.The results of morphological characteristics and phylogenetic analyses with internal transcribed spacer(ITS),calmodulin(CAM)and translation elongation factor-1α(TEF-1α)showed that these isolates classified into three groups,Fusarium sulawesiense(36.36%),F.pernambucanum(36.36%),and F.citri(27.28%).Further pathogenicity tests were performed using three isolates TEY9-2,TEY12-2,and TEY10A-3,each representing one of the Fusarium species and all of the test isolates could cause the same symptoms of leaf blight on plum under wounded conditions in which F.citri is the first report causing leaf blight on plum in China.

关 键 词：PLUM leaf blight Fusarium sulawesiense F.pernambucanum F.citri 

分 类 号：S432.44[农业科学—植物病理学]