Uncovering the prominent role of satellite cells in paravertebral muscle development and aging by single-nucleus RNA sequencing  

作　　者：Xin Qiu Hao-Yu Wang Zhen-Yu Yang Li-Ming Sun Shu-Nan Liu Chui-Qin Fan Feng Zhu 

机构地区：[1]Department of Spinal Surgery,The University of Hong Kong-Shenzhen Hospital,Shenzhen,Guangdong 518000,China [2]Department of Orthopedics and Traumatology,Li Ka Shing Faculty of Medicine,The University of Hong Kong,Hong Kong SAR 999077,China [3]College of Life Sciences,University of Chinese Academy of Sciences,Beijing 100000,China [4]Lars Bolund Institute of Regenerative Medicine,Qingdao-Europe Advanced Institute for Life Sciences,BGI-Qingdao,Qingdao,Shandong 266000,China [5]China Medical University,Shenyang,Liaoning 110000,China [6]Department of Plastic and Reconstructive Surgery,Xijing Hospital,Forth Military Medical University,Xi'an,Shaanxi 710000,China

出　　处：《Genes & Diseases》2023年第6期2597-2613,共17页基因与疾病（英文）

基　　金：supported by the Shenzhen Fundamental Research Program,China(No.JCYJ20180306173518936);Sanming Project of Medicine in Shenzhen,China(No.SZSM201612055);HKU-SZH Fund for Shenzhen Key Medical Discipline(China)(No.SZXK2020084);China National GeneBank(CNGB).

摘　　要：To uncover the role of satellite cells(SCs)in paravertebral muscle development and aging,we constructed a single-nucleus transcriptomic atlas of mouse paravertebral muscle across seven timepoints spanning the embryo(day 16.5)to old(month 24)stages.Eight cell types,including SCs,fast muscle cells,and slow muscle cells,were identified.An energy metabolism-related gene set,TCA CYCLE IN SENESCENCE,was enriched in SCs.Forty-two skeletal muscle disease-related genes were highly expressed in SCs and exhibited similar expression patterns.Among them,Pdha1 was the core gene in the TCA CYCLE IN SENESCENCE;Pgam2,Sod1,and Suclg1 are transcription factors closely associated with skeletal muscle energy metabolism.Transcription factor enrichment analysis of the 42 genes revealed that Myod1 and Mef2a were also highly expressed in SCs,which regulated Pdha1 expression and were associated with skeletal muscle development.These findings hint that energy metabolism may be pivotal in SCs development and aging.Three ligand-receptor pairs of extracellular matrix(ECM)-receptor interactions,Lamc1-Dag1,Lama2-Dag1,and Hspg2-Dag1,may play a vital role in SCs interactions with slow/fast muscle cells and SCs self-renewal.Finally,we built the first database of a skeletal muscle single-cell transcriptome,the Musculoskeletal Cell Atlas(http://www.mskca.tech),which lists 630,040 skeletal muscle cells and provides interactive visualization,a useful resource for revealing skeletal muscle cellular heterogeneity during development and aging.Our study could provide new targets and ideas for developing drugs to inhibit skeletal muscle aging and treat skeletal muscle diseases.

关 键 词：NICHE Paraspinal muscle Satellite cells SENESCENCE Single-nucleus RNA sequencing 

分 类 号：R73[医药卫生—肿瘤]