Dihydroartemisinin increased the abundance of Akkermansia muciniphila by YAP1 depression that sensitizes hepatocellular carcinoma to anti-PD-1 immunotherapy  

作　　者：Zhiqin Zhang Xinli Shi Jingmin Ji Yinglin Guo Qing Peng Liyuan Hao Yu Xue Yiwei Liu Caige Li Junlan Lu Kun Yu 

机构地区：[1]Department of Pathobiology and Immunology,Hebei University of Chinese Medicine,Shijiazhuang 050200,China

出　　处：《Frontiers of Medicine》2023年第4期729-746,共18页医学前沿(英文版)

基　　金：the National Natural Science Foundation of China(No.81873112);Science and Technology Project of Hebei Education Department(No.ZD2022120)for the economic support.

摘　　要：The effect of anti-programmed cell death 1(anti-PD-1)immunotherapy is limited in patients with hepatocellular carcinoma(HCC).Yes-associated protein 1(YAP1)expression increased in liver tumor cells in early HCC,and Akkermansia muciniphila abundance decreased in the colon.The response to anti-PD-1 treatment is associated with A.muciniphila abundance in many tumors.However,the interaction between A.muciniphila abundance and YAP1 expression remains unclear in HCC.Here,anti-PD-1 treatment decreased A.muciniphila abundance in the colon,but increased YAP1 expression in the tumor cells by mice with liver tumors in situ.Mechanistically,hepatocyte-specific Yap1 knockout(Yap1^(LKO))maintained bile acid homeostasis in the liver,resulting in an increased abundance of A.muciniphila in the colon.Yap1 knockout enhanced anti-PD-1 efficacy.Therefore,YAP1 inhibition is a potential target for increasing A.muciniphila abundance to promote anti-PD-1 efficacy in liver tumors.Dihydroartemisinin(DHA),acting as YAP1 inhibitor,increased A.muciniphila abundance to sensitize anti-PD-1 therapy.A.muciniphila by gavage increased the number and activation of CD8^(+)T cells in liver tumor niches during DHA treatment or combination with anti-PD-1.Our findings suggested that the combination anti-PD-1 with DHA is an effective strategy for liver tumor treatment.

关 键 词：hepatocellular carcinoma YAP1 Akkermansia muciniphila anti-PD-1 DIHYDROARTEMISININ bile acid 

分 类 号：R735.7[医药卫生—肿瘤]