低温风干发酵羊腿中优势乳酸菌的分离、筛选及鉴定  

Isolation,Screening and Identification of Dominant Lactic Acid Bacteria from Air-Dried Sheep Legs Fermented at Low Temperature

作　　者：马依努尔·米吉提 茹克叶木·阿不力米提 力俊琛 巴吐尔·阿不力克木 Maynur Mijit;Rukeyemu Ablikim;LI Junchen;Batuer Abulikemu(College of Food Science and Pharmacy,Xinjiang Agricultural University,Urumqi 830052,Xinjiang,China)

机构地区：[1]新疆农业大学食品科学与药学学院,新疆乌鲁木齐830052

出　　处：《食品研究与开发》2023年第21期152-159,共8页Food Research and Development

基　　金：新疆维吾尔自治区农业农村厅2021自治区畜牧业生产发展专项(xjnqry-g-2008)。

摘　　要：为获得发酵特性优良和性质稳定的香肠发酵菌种,以低温风干发酵羊腿为试验材料,采用溶钙圈法分离、筛选发酵特性良好的菌株,并对菌株产酸能力以及在不同条件下的生存能力进行复筛,根据形态学和生理生化特征结合16S rDNA序列分析进行鉴定。结果表明,从低温风干发酵羊腿中分离纯化得到43株乳酸菌,其中GM09、GM23、GM28、PA15和KN02 5株乳酸菌发酵特性好,具有良好的产酸能力、发酵和生长特性。经生理生化及分子生物学鉴定GM09、PA15为戊糖乳杆菌(Lactobacillus pentosus),GM23、GM28、KN02为植物乳杆菌(Lactobacillus plantarum)。The strains with good fermentation characteristics and stable properties were isolated and screened from the air-dried sheep legs fermented at low temperature by the calcium-dissolving circle method.The strains preliminarily screened out were rescreened based on the acid production and survival under different conditions.The strains were identified according to morphological,physiological,and biochemical characteristics and16S rDNA sequence analysis.The results showed that 43 strains of lactic acid bacteria were isolated from lowtemperature air-dried fermented sheep legs.Among them,five strains,GM09,GM23,GM28,PA15,and KN02,had excellent fermentation characteristics,high acid production,and good growth performance.GM09and PA15 were identified as Lactobacillus pentosus,and GM23,GM28,and KN02 as L.plantarum.

关 键 词：风干羊腿 乳酸菌 筛选 鉴定 发酵特性 

分 类 号：TS251.1[轻工技术与工程—农产品加工及贮藏工程]