发酵桔皮对酿酒酵母生长性能的影响研究  

Study on Effect of Fermented Orange Peel on Yeast Growth Performance

作　　者：马吉喆 吴雨来 张林梅 叶倩颖 陆筑凤[2] 李加友[2] 徐涛[1] MA Jizhe;WU Yulai;ZHANG Linmei;YE Qianying;LU Zhufeng;LI Jiayou;XU Tao(College of Life Science and Medicine,Zhejiang Sci-Tech University,Zhejiang Hangzhou 310018,China;School of Biological and Chemical Engineering,Jiaxing University,Zhejiang Jiaxing 314001,China)

机构地区：[1]浙江理工大学生命科学与医药学院,浙江杭州310018 [2]嘉兴学院生物与化学工程学院,浙江嘉兴314001

出　　处：《饲料工业》2023年第21期94-101,共8页Feed Industry

基　　金：浙江省自然科学基金公益项目[LGN22C200009];嘉兴市科技计划项目[2021AD10010]。

摘　　要：研究旨在通过添加新型生长刺激剂促进酵母生长,提升酵母的生长效率。通过向酵母培养基中添加发酵桔皮,探究发酵桔皮对酵母生长的影响,利用单因素试验、正交试验、响应面分析等方法对添加发酵桔皮的酵母培养基进行优化。结果表明:添加发酵桔皮后的优化培养基为:葡萄糖100.756 g/L、蛋白胨8 g/L、酵母浸膏11.906 g/L、发酵桔皮(干物质含量)1.906 g/L、无水硫酸镁2 g/L、尿素2 g/L、磷酸二氢钾5 g/L。培养基优化后酵母生物量达到最大,比未添加发酵桔皮的培养基提高了1.3倍。发酵桔皮可以作为酵母生长刺激剂提高酵母活菌浓度,缩短酵母进入对数生长期的时间,从而促进酵母饲料工业降本增效。The research aims to promote yeast growth and improve yeast growth efficiency by adding new growth stimulants.By adding fermented orange peel to explore the effect of fermented orange peel on yeast growth in yeast medium.Next,single-factor experiments,orthogonal experiments,response surface analysis and other methods were used to optimize the yeast medium with fermented orange peel.The results showed that the optimized medium after adding fermented orange peel was:C6H12O6100.756 g/L,peptone 9 g/L,yeast extract 11.906 g/L,fermented orange peel(dry matter content)1.906 g/L,MgSO42 g/L,urea 2 g/L,KH2SO45 g/L.After medium optimization,yeast biomass was maximized,which was 1.3 times higher than medium without fermented orange peel.Fermented orange peel can be used as yeast growth stimulants to increase yeast live bacteria concentration,shorten the time for yeast to enter the logarithmic growth period,thereby reducing costs and increasing efficiency for yeast feed industry.

关 键 词：桔皮 发酵 酵母 生长刺激剂 酵母饲料 

分 类 号：S816.6[农业科学—饲料科学]