玉米皮中阿魏酸超声辅助碱醇提取及大孔树脂纯化工艺优化  

Optimization of Ultrasound-Assisted Alkali Alcohol Extraction and Macroporous Resin Purification of Ferulic Acid from Corn Husk

作　　者：张乃丹 佐兆杭 王颖[1,2,3,4] 孙维 庞惟俏[1] ZHANG Naidan;ZUO Zhaohang;WANG Ying;SUN Wei;PANG Weiqiao(College of Food,Heilongjiang Bayi Agricultural University,Daqing 163319,China;National Coarse Cereals Engineering Research Center,Daqing 163319,China;Engineering Research Center of Processing and Utilization of Grain By-products,Ministry of Education,Daqing 163319,China;Key Laboratory of Agro-Products Processing and Quality Safety of Heilongjiang Province,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学食品学院,黑龙江大庆163319 [2]国家杂粮工程技术中心,黑龙江大庆163319 [3]粮食副产物加工与利用教育部工程研究中心,黑龙江大庆163319 [4]黑龙江省农产品加工与质量安全重点实验室,黑龙江大庆163319

出　　处：《食品工业科技》2023年第22期1-11,共11页Science and Technology of Food Industry

基　　金：国家重点研发计划项目“杂粮产业链一体化示范”(2020YFD1001400);国家重点研发计划项目“杂粮食品精细化加工关键技术合作研究及应用示范”(2018YFE0206300);黑龙江省自然科学基金研究团队项目“杂粮与主粮复配科学基础及慢病干预机制”(TD2020C003);黑龙江省省属高等学校基本科研业务费科研项目“杂粮主食化食品生产关键技术与高附加值产品开发”(ZDZX202106)。

摘　　要：为探究玉米皮中阿魏酸的提取、纯化工艺。以玉米皮为原料,采用超声辅助碱醇法提取玉米皮中阿魏酸,通过单因素实验和响应面优化试验,以粗提液中阿魏酸的含量为指标得出最佳提取工艺条件。进一步采用大孔吸附树脂法对玉米阿魏酸进行纯化,通过静态吸附方法选出适合树脂进行实验。在动态吸附方法中确定最佳上样量,在通过单因素实验和响应面优化试验,以回收率为指标得出最佳纯化工艺条件。结果表明,玉米皮中阿魏酸最佳提取工艺条件为:料液比1:12 g/mL,碱液质量浓度4%,碱醇比2:1,超声时间30 min,超声温度59℃,超声功率229 W,此条件下阿魏酸的提取量可达22.31 mg/g。阿魏酸纯化部分采用HPD-100型大孔吸附树脂,最佳上样量为6倍床体积,最佳纯化工艺条件为:上样浓度0.3 mg/mL,上样流速3 mL/min,乙醇质量浓度75%,洗脱流速1 mL/min。此条件下玉米皮阿魏酸的回收率可达95.17%,阿魏酸纯度为81.56%。本实验增加了玉米皮附加值,为玉米皮深加工和阿魏酸开发提供理论依据和数据支持。To explore the extraction and purification process of ferulic acid from corn bran,ferulic acid from corn husks was extracted by ultrasound-assisted alkali alcohol method.The best extraction conditions were obtained by using the content of ferulic acid in the crude extract of corn husk as the index through single factor tests and response surface optimization test.Further purification of corn ferulic acid was used macroporous adsorption resin method,and suitable resin was selected by static adsorption method.Determined the optimal sample loading amount in the dynamic adsorption method,and obtained the optimal purification process conditions using recovery rate as an indicator through single factor experiments and response surface optimization experiment.Results showed that,the best extraction conditions of ferulic acid from corn bran were obtained as follows:Solid-liquid ratio 1:12 g/mL,mass concentration of lye was 4%,alkali-alcohol ratio was 2:1,ultrasound time 30 min,ultrasonic thermometer 59℃,ultrasonic power 229 W,and the amount of ferulic acid extracted could reached 22.31 mg/g under these conditions.The purification of ferulic acid was performed using HPD-100 macroporous adsorption resin.The optimal loading volume was 6 times the bed volume.The optimal purification process conditions are as follows:Sample loading concentration 0.3 mg/mL,loading flow rate 3 mL/min,mass concentration of ethanol 75%,elution flow rate 1 mL/min.Under these conditions,the recovery rate of ferulic acid from corn peel was up to 95.17%,and the purity of ferulic acid was 81.56%.This experiment increased the added value of corn brans,and would provide the theoretical basis and data support for the further processing of corn brans and the development of ferulic acid.

关 键 词：玉米皮 阿魏酸 超声辅助碱醇法 大孔吸附树脂法 工艺优化 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]