Hemorrhagic transformation in patients with large-artery atherosclerotic stroke is associated with the gut microbiota and lipopolysaccharide  

作　　者：Qin Huang Minping Wei Xianjing Feng Yunfang Luo Yunhai Liu Jian Xia 

机构地区：[1]Department of Neurology,Xiangya Hospital,Central South University,Changsha,Hunan Province,China [2]Department of Neurology,Peking University People’s Hospital,Beijing,China [3]Clinical Research Center for Cerebrovascular Disease of Hunan Province,Central South University,Changsha,Hunan Province,China [4]National Clinical Research Center for Geriatric Disorders,Xiangya Hospital,Central South University,Changsha,Hunan Province,China

出　　处：《Neural Regeneration Research》2024年第7期1532-1540,共9页中国神经再生研究（英文版）

基　　金：supported by the National Key Research and Development Projects,Nos.2022 YFC3602400,2022 YFC3602401(to JX);the Project Program of National Clinical Research Center for Geriatric Disorders(Xiangya Hospital),No.2020LNJJ16(to JX);the National Natural Science Foundation of China,No.82271369(to JX)。

摘　　要：Hemorrhagic transformation is a major complication of large-artery atheroscle rotic stroke(a major ischemic stro ke subtype)that wo rsens outcomes and increases mortality.Disruption of the gut microbiota is an important feature of stroke,and some specific bacteria and bacterial metabolites may contribute to hemorrhagic transformation pathogenesis.We aimed to investigate the relationship between the gut microbiota and hemorrhagic transformation in largearte ry atheroscle rotic stro ke.An observational retrospective study was conducted.From May 2020 to September 2021,blood and fecal samples were obtained upon admission from 32 patients with first-ever acute ischemic stroke and not undergoing intravenous thrombolysis or endovascular thrombectomy,as well as 16 healthy controls.Patients with stro ke who developed hemorrhagic transfo rmation(n=15)were compared to those who did not develop hemorrhagic transformation(n=17)and with healthy controls.The gut microbiota was assessed through 16S ribosomal ribonucleic acid sequencing.We also examined key components of the lipopolysaccharide pathway:lipopolysaccharide,lipopolysaccharide-binding protein,and soluble CD14.We observed that bacterial diversity was decreased in both the hemorrhagic transformation and non-hemorrhagic transfo rmation group compared with the healthy controls.The patients with ischemic stro ke who developed hemorrhagic transfo rmation exhibited altered gut micro biota composition,in particular an increase in the relative abundance and dive rsity of members belonging to the Enterobacteriaceae family.Plasma lipopolysaccharide and lipopolysaccharide-binding protein levels were higher in the hemorrhagic transformation group compared with the non-hemorrhagic transfo rmation group.lipopolysaccharide,lipopolysaccharide-binding protein,and soluble CD14 concentrations were associated with increased abundance of Enterobacte riaceae.Next,the role of the gut microbiota in hemorrhagic transformation was evaluated using an experimental stroke rat model.In this model,t

关 键 词：gut microbiota hemorrhagic transformation INFLAMMATION LIPOPOLYSACCHARIDE STROKE 

分 类 号：R743.3[医药卫生—神经病学与精神病学]