假节杆菌PL-410产软骨素裂解酶制备条件优化  

Optimization of Fermentation Conditions for Chondroitinase Production by Pseudarthrobacter sp.PL-410

作　　者：肖梦圆 李平兰[1] 武瑞赟[1,2] XIAO Mengyuan;LI Pinglan;WU Ruiyun(College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China;Integrated Laboratory of Processing Technology for Chinese Meat and Dish Products,Ministry of Agriculture and Rural Affairs,Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]中国农业科学院农产品加工研究所,农业农村部中式肉类菜肴制品加工技术集成实验室,北京100193

出　　处：《肉类研究》2023年第9期21-29,共9页Meat Research

基　　金：现代农业产业技术体系北京市渔业创新团队项目(BAIC07-2023-13)。

摘　　要：为确定获得软骨素裂解酶产生菌的分类地位及提高菌株产酶水平,通过形态学和16S rRNA基因序列分析鉴定菌株PL-410,并采用单因素试验、Plackett-Burman试验、最陡爬坡试验和Box-Behnken响应面试验优化菌株PL-410的产酶条件。结果表明,菌株PL-410为假节杆菌属(Pseudarthrobacter sp.),命名为假节杆菌PL-410;优化的最佳培养基为牛软骨硫酸软骨素添加量7.5 g/L、胰蛋白胨添加量2.5 g/L、NaCl添加量5 g/L、KH2PO4添加量0.3 g/L、培养基初始pH 6.53,最佳发酵条件为接种量2.98%、摇瓶装液量8%、摇床转速160 r/min、发酵温度24℃、发酵时间27.63 h,在此条件下软骨素裂解酶的活力(2531.765 U/L)比优化前(85.229 U/L)提高28.7倍,可更有效地降解牛软骨硫酸软骨素。In order to determine its taxonomic status and to improve its enzyme production level,the chondroitinaseproducing strain PL-410 was identified based on morphology and 16S rRNA gene sequence analysis,and the fermentation conditions for chondroitinase production by this strain were optimized by single factor experiments,Plackett-Burman design,the steepest ascent method and Box-Behnken design combined with response surface methodology.The results showed that the strain PL-410 was identified as Pseudarthrobacter sp.The optimal medium was composed of chondroitin sulfate from bovine cartilage 7.5 g/L,tryptone 2.5 g/L,NaCl 5 g/L,KH2PO40.3 g/L,and initial pH 6.53.The optimal fermentation conditions were as follows:inoculum volume 2.98%,medium volume in shaking flasks 8%,shaker rotation speed 160 r/min,fermentation temperature 24℃,and fermentation time 27.63 h.The chondroitinase activity under these conditions was 2531.765 U/L,which was 29.7 times higher than that before optimization(85.229 U/L).

关 键 词：假节杆菌 软骨素裂解酶 产酶条件优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]