肽酰精氨酸脱氨酶4和组蛋白H3瓜氨酸化与高级别脑胶质瘤的相关性研究  

Correlation research of peptidyl arginine deaminase 4 and histone H3 citrullination and high-grade glioma

作　　者：孔晨旭 李振江[1] 耿慧霞 王来[3] KONG Chenxu;LI Zhenjiang;GENG Huixia;WANG lai(Huaihe Hospital of Henan University,Kaifeng 475004,CHN;School of Nursing and Health,Henan University,Kaifeng 476000,CHN;School of Life Sciences,Henan University,Kaifeng 476000,CHN)

机构地区：[1]河南大学淮河医院,河南开封475004 [2]河南大学护理与健康学院,河南开封476000 [3]河南大学生命科学学院,河南开封476000

出　　处：《河南大学学报（医学版）》2023年第5期346-349,共4页Journal of Henan University:Medical Science

基　　金：国家自然科学基金(81871856);河南省科技攻关计划(222102310434)。

摘　　要：目的:研究肽酰精氨酸脱氨酶4和组蛋白H3瓜氨酸化的表达与脑胶质瘤分级的关系。方法:选取2020年1月—2022年1月在河南大学淮河医院进行手术治疗的脑胶质瘤患者30例为脑胶质瘤组,其中18例为Ⅲ级、12例为Ⅳ级,取手术计划切除范围内正常脑组织为对照组。免疫组织化学检测肽酰精氨酸脱氨酶4和组蛋白H3瓜氨酸化的表达情况,并分析其与脑胶质瘤分级的关系。结果:与正常组织相比较,Ⅲ级和Ⅳ级胶质瘤组织,肽酰精氨酸脱氨酶4和组蛋白H3瓜氨酸化的表达水平上调,且上调程度与胶质瘤的级别成正比。结论:肽酰精氨酸脱氨酶4和组蛋白H3瓜氨酸化在胶质瘤组织高表达,且表达水平与级别相关,提示PAD4介导的组蛋白瓜氨酸化参与脑胶质瘤的发展。Objective:To study the relationship between the expression of peptidyl arginine deaminase 4 and histone H3 citrullination and high-grade glioma.Methods:The 30 glioma patients who underwent surgical treatment in Huaihe Hospital of Henan University were selected as the glioma group from January 2021to January 2022,of which 18 cases were grade Ⅲ and 12 cases were grade Ⅳ,and the normal brain tissue within the surgical plan were taken as the control group.The expressions of peptidyl arginine deaminase 4 and histone H3 citrullination were detected by immunohistochemistry,and their relationship with the grade of glioma was analyzed.Results:Compared with the normal tissues,the expression levels of peptidyl arginine deaminase 4 and histone H3 citrullination were up-regulated in grade Ⅲ and grade Ⅳ glioma tissues,and the up-regulation degree was proportional to the grade of glioma.Conclusion:Peptidyl arginine deaminase 4 and histone H3 citrullination are highly expression levels in glioma tissues,and the expression levels are correlated with grades,suggesting that PAD4-mediated histone citrullination is involved in the development of glioma.

关 键 词：脑胶质瘤 分级 肽酰精氨酸脱氨酶4 瓜氨酸化 

分 类 号：R739.41[医药卫生—肿瘤]