层析柱洗脱条件对保健品中总皂苷含量测定的影响  

Influence of Chromatography Column Elution Conditions on the Determination of Total Saponins in Health Products on Coefficient of Variation Method

作　　者：杜晶 陈昭 杨子忠 李亚军 吴媛媛 甘琴 冯雪萍 DU Jing;CHEN Zhao;YANG Zizhong;LI Yajun;WU Yuanyuan;GAN Qin;FENG Xueping(Hubei Provincial Yichang Angel Nutritech Co.,Ltd.,Yichang,Hubei 443003,China)

机构地区：[1]湖北省宜昌市安琪纽特股份有限公司,湖北宜昌443003

出　　处：《农产品加工》2023年第19期65-67,72,共4页Farm Products Processing

摘　　要：为建立紫外分光光度法测定保健食品苦瓜硒铬酵母胶囊中总皂苷含量的方法。以Amberlite-XAD-2型大孔树脂和中性氧化铝作为层析柱,采用水-乙醇溶剂系统对样品的皂苷成分进行提取、纯化、富集和洗脱,人参皂苷Re作为对照品,以高氯酸显色后利用紫外分光光度法于波长560 nm处测定其总皂苷含量,并进行方法学考查。通过对前处理进行优化,以70%乙醇提取、吸附30 min并采用20 m L水进行洗脱,总皂苷提取含量为1.596%,可达最高。通过线性、回收率和精密度进行方法学考查,人参皂苷Re在0~0.167 mg/m L的质量浓度范围内呈良好线性关系,相关系数R~2为0.999 5;总皂苷加标回收率为100.8%~104.6%,平均回收率为102.6%,RSD为1.5%;精密度试验平均含量为1.09%,RSD为2.8%,表明保健品总皂苷的提取方法优化使得提取率高且稳定可行。此方法操作简单,准确性好、重复性好,适用于保健食品中总皂苷的含量测定。To establish a method for the determination of total saponins in dietary supplement momordica charantia selenium-chromium yeast capsules by UV Spectrophotometer.Using Amberlite-XAD-2 macroporous resin and neutral alumina as chromatographic column,the saponins were extracted,purified,enriched and eluted by water-ethanol solvent system.Ginsenoside Re was used as control,the content of total saponins was determined by UV Spectrophotometer at 560 nm after developing with perchloric acid.The results showed that the extraction content of total saponins was 1.596%,which was the highest by optimizing the pretreatment,extracting with 70%ethanol,adsorbing for 30 min and eluting with 20 ml water.The linearity,recovery and precision of ginsenoside Re showed a good linear relationship in the range of 0~0.167 mg/mL,and the correlation coefficient R2 was 0.9995;the average recoveries of total saponins were 100.8%~104.6%,the average recoveries were 102.6%and RSD were 1.5%,the average contents of total saponins were 1.09%and RSD were 2.8%,the results showed that the optimization of the extraction method of total saponins made the extraction rate high,stable and feasible.The method was simple,accurate and reproducible,and was suitable for the determination of total saponins in dietary supplement.

关 键 词：人参皂苷RE 紫外分光光度计法 保健食品 检测 

分 类 号：R151.3[医药卫生—营养与食品卫生学]