3-羟基丁酸对雨生红球藻PSⅡ光化学活性与色素合成的影响  

Effects of 3-Hydroxybutyric Acid on PSⅡPhotochemical Activity and Pigment Synthesis in Haematococcus pluvialis

作　　者：黄莹 张娟 窦勇 原雪峰 翟胜利 曲木 周文礼 HUANG Ying;ZHANG Juan;DOU Yong;YUAN Xuefeng;ZHAI Shengli;QU Mu;ZHOU Wenli(Tianjin Key Laboratory for Aquaculture Ecology and Cultivation,Fisheries College of Tianjin Agriculture University,Tianjin 300384,China;Tianjin Modern Tianjiao Agricultural Science and Technology Co.,Ltd.,Tianjin Key Laboratory for Green and Ecological Forage,Tianjin 301801,China;Tianjin Chenhui Technology Group,Tianjin 301802,China)

机构地区：[1]天津农学院水产学院,天津市水产生态及养殖重点试验室,天津300384 [2]天津现代天骄农业科技股份有限公司,天津市绿色生态饲料重点实验室,天津301801 [3]天津现代晨辉科技集团,天津301802

出　　处：《食品研究与开发》2023年第22期7-13,共7页Food Research and Development

基　　金：天津市技术创新引导专项(基金)(20YDTPJC01470);中央引导地方科技发展资金(22ZYCGSN00010);天津市水产生态及养殖重点实验室开放基金项目(TJAE201805);自然资源部海洋生态系统动力学重点实验室开放基金项目(MED202013);天津市农业科技成果转化与推广项目(202001010);天津市宝坻区农业科技计划-特派员帮扶项目(202012);天津市科技局生态环境治理重大专项(18ZXSZSF00080)。

摘　　要：利用试验生态学方法,研究3-羟基丁酸对雨生红球藻光系统Ⅱ(photosystemⅡ,PSⅡ)光化学活性与色素合成的影响。结果发现,3-羟基丁酸对雨生红球藻PSⅡ光反应中心造成胁迫损伤,细胞PSⅡ光化学最大光能转化效率(Fv/Fm)、有效光能转化效率(Yield)和表观电子传递速率(electron transfer rate,ETR)的参数均出现不同程度降低,其中0.02、0.10μg/L处理组的作用最明显;3-羟基丁酸造成雨生红球藻叶绿素a含量波动变化并最终下降至较低水平;3-羟基丁酸能够促进雨生红球藻合成并积累虾青素,其中0.01μg/L浓度的促进作用最强,虾青素最高产量为7.57 mg/L;3-羟基丁酸影响了雨生红球藻自身资源在光合作用与抗逆合成之间的权衡分配。研究结果为使用3-羟基丁酸作为外源诱导物促进雨生红球藻生产虾青素提供了依据。Using the experimental ecological method,the study investigated the effects of 3-hydroxybutyric acid on photosystemⅡ(PSⅡ)photochemical activity and pigment synthesis in Haematococcus pluvialis.The results revealed that 3-hydroxybutyric acid caused stress-induced damage to the PSⅡphotoreaction centers of the H.pluvialis.PSⅡphotochemical parameters,including maximum light energy conversion efficiency(Fv/Fm),effective light energy conversion efficiency(Yield),and apparent electron transfer rate(ETR),were all reduced to varying degrees,with the most significant effects observed in the groups treated with 3-hydroxybutyric acid at 0.02 and 0.10μg/L.Furthermore,3-hydroxybutyric acid led to fluctuating changes in chlorophyll a content in H.pluvialis and eventually decreased it to a lower level.Additionally,3-hydroxybutyric acid promoted the synthesis and accumulation of astaxanthin in H.pluvialis,with the most potent effect observed at a concentration of 0.01μg/L,resulting in the highest astaxanthin yield of 7.57 mg/L.Overall,3-hydroxybutyric acid affected the allocation of resources in H.pluvialis between photosynthesis and stress resistance synthesis.The findings provided a basis for using 3-hydroxybutyric acid as an exogenous inducer to promote astaxanthin production in H.pluvialis.

关 键 词：3-羟基丁酸 雨生红球藻 光系统Ⅱ光化学活性 叶绿素A 虾青素 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]