三种分子分型方法对2018年辽宁省副溶血性弧菌的分型研究  

Comparative study on three molecular typing methods of Vibrio parahaemolyticus

作　　者：李雪[1] 孙婷婷[1] 魏彤竹[1] 王伟杰[1] 刁文丽[1] Li Xue;Sun Tingting;Wei Tongzhu;Wang Weijie;Diao Wenli(Liaoning Provincial Center for Disease Control and Prevention,Shenyang 110005,China)

机构地区：[1]辽宁省疾病预防控制中心,沈阳110005

出　　处：《卫生研究》2023年第6期956-964,共9页Journal of Hygiene Research

基　　金：辽宁省自然科学基金(No.2018-ZD-1091)。

摘　　要：目的评价脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)、基因外重复回文系列(repetitive extragenic palindromic,REP)-PCR和肠杆菌基因间保守重复序列(en-terobacterial repetitive intergenic consensus,ERIC)-PCR三种分子分型方法间的关联性,同时探讨菌株间的亲缘关系,并结合血清型分析,进一步了解辽宁省副溶血性弧菌的分布规律及流行趋势。方法对2018年辽宁省150株副溶血性弧菌分离株进行血清分型、PFGE、REP-PCR和ERIC-PCR分子分型和聚类分析。结果118株分离株共分为14个血清型,32株分离株未能分型,血清群以O3群、O1群和O2群为主。PFGE的分辨力(DI)为0.969,REP-PCR的分辨力(DI)为0.948,ERIC-PCR的分辨力(DI)为0.927。血清型O3群菌株与O1群菌株分子型相似度高。结论2018年辽宁省临床副溶血性弧菌分离株流行血清型仍为O3:K6,食品副溶血性弧菌分离株流行血清群仍为O2。PFGE、REP-PCR和ERIC-PCR三种分型方法结果一致,且PFGE分型方法的分辨力和再现性均优于其他两种方法。血清型O3群与O1群菌株密切相关。OBJECTIVE To evaluate the correlation among the three molecular typing method of pulsed field gel electrophoresis(PFGE),repetitive extragenic palindromic(REP)-PCR and en-terobacterial repetitive intergenic consensus(ERIC)-PCR,and to explore the genetic relationship among strains,and to further understand the distribution and epidemic trend of Vibrio parahaemolyticus in Liaoning Province by combining Serotype analysis.METHODS Serum typing,PFGE,REP-PCR,and ERICPCR molecular typing and cluster analysis were performed on 150 VP isolates from Liaoning Province in 2018.RESULTS 118 isolates could be divided into 14 Serotype,and 32 isolates could not be classified.The main serotypes were O3,O1 and O2.The resolution(DI)of PFGE is 0.969,the resolution(DI)of REP-PCR is 0.948,and the resolution(DI)of ERIC-PCR is 0.927.The Serotype O3 group strains are highly similar to the molecular types of O1 group strains.CONCLUSION In 2018,the epidemic Serotype of clinical VP isolates in Liaoning Province is still O3:K6,and the epidemic serotype of food VP isolates is still O2.The result of PFGE,REP-PCR,and ERIC-PCR typing method are consistent,and the resolution and reproducibility of PFGE typing method are superior to the other two method.The Serotype O3 group is closely related to O1 group.

关 键 词：副溶血性弧菌 分子分型 血清分型 亲缘关系 溯源 

分 类 号：R155.5[医药卫生—营养与食品卫生学]