甜菜及其近缘类群叶绿体基因组重复序列比较分析  

Comparative Analysis of Chloroplast Genome Repeats of Beta vulgaris and Its Related Groups

作　　者：包国媛 王雅琼 李文辛 BAO Guoyuan;WANG Yaqiong;LI Wenxin(College of Ecology,Environment and Resources,Qinghai Minzu University,Xining 810007,China;Qinghai Key Laboratory of Biotechnology and Analytical Testing,Xining 810007,China;Qinghai Provincial Key Laboratory of High-Value Utilization of Characteristic Economic Plants,Xining 810007,China;State Ethnic Affairs Commission Key Laboratory of Resource Chemistry and Eco-Environmental Protection on Qinghai-Tibet Plateau,Xining 810007,China)

机构地区：[1]青海民族大学生态环境与资源学院,西宁810007 [2]青海省生物技术与分析测试重点实验室,西宁810007 [3]青海省特色经济植物高值化利用重点实验室,西宁810007 [4]青藏高原资源化学与生态环境保护国家民委重点实验室,西宁810007

出　　处：《种子》2023年第9期36-44,共9页Seed

基　　金：青海民族大学2023年大学生创新创业训练计划项目:“甜”酒佳酿——做青海省红甜菜酿造酒拓荒者;青海民族大学研究生创新项目(54M2022005);2023年中央科研创新平台建设项目——青海省林业草原生态系统功能维护及可持续开发利用科研创新团队(30160101141)。

摘　　要：甜菜、菠菜及藜麦具有较高的食用、药用和饲用价值。通过MISA和REPuter软件对其叶绿体全基因组的简单重复序列(cpSSR)和散在重复序列(cpIRS)进行分析,研究甜菜及其近缘类群的叶绿体基因组重复序列差异。结果表明,甜菜及其近缘类群的全长分别为149722 bp、150725 bp和152079 bp,均由一个大单拷贝区(LSC)(83110 bp、82719 bp、83551 bp),一个小单拷贝区(SSC)(17793 bp、17860 bp、18118 bp)和一对反向重复序列(IRs)(24410 bp、25073 bp、25205 bp)组成。甜菜及其近缘类群叶绿体基因组中共检测到195个,171个,154个SSR位点,平均每隔767.8 bp、881.4 bp、987.5 bp出现一个SSR位点。重复序列中主要以单碱基重复序列为主,分别占SSR位点总数的91.8%,85.35%,89.6%。cpSSR主要集中分布在大单拷贝区(LSC),占SSR位点总数的67.69%,68.82%及65.59%;cpSSR长度主要集中在8~12 bp之间,说明叶绿体基因组上的微卫星位点大多数具有较高的多态性。REPuter共检测到甜菜、菠菜及藜麦存在50个,13个及25个cpIRS序列,甜菜中检测到26个正向重复(F型)和24个回文重复(P型),菠菜和藜麦中只检测到1种重复类型,均正向重复(F型)。Beta vulgaris,Spinacia oleracea and Chenopodium quinoa Willd have high edible,medicinal and feeding values.In order to study the chloroplast genome repeats sequence difference between beet and its related groups,MISA and REPuter were used to analyze the complete chloroplast whole genome cpSSR and cpIRS of beet and its related groups.The results showed that the total length of Beta vulgaris and its related groups were 149722 bp,150725 bp and 152079 bp,respectively,and all of them were consisted by a single LSC(83110 bp,82719 bp,83551 bp),a single SSC(17793 bp,17860 bp,18118 bp)and a pair of IRs(24410 bp,25073 bp,25205 bp)a single.A total of 195,171 and 154 SSR loci were detected in the chloroplast genome of Beta vulgaris and related groups,with an average of one SSR loci every 767.8 bp,881.4 bp and 987.5 bp.Single base repeats were dominant,accounting for 91.8%,85.35%and 89.6%of the total SSR loci,respectively.cpSSR mainly distributed in LSC region,accounting for 67.69%,68.82%and 65.59%of the total number of SSR loci.The length of cpSSR was mainly between 8 and 12 bp,indicating that most microsatellite loci on chloroplast genome had high polymorphism.Beta vulgaris and its related taxa were detected in REPuter with 50,13 and 25 cpIRS sequences,26 direct(F type)and 24 inverted repeats(P type)were detected in Beta vulgaris and only one type of repeat was detected in Spinacia oleracea and Chenopodium quinoa Willd,all of which were direct repeat(F type).

关 键 词：甜菜 叶绿体基因组 重复序列 CPSSR 

分 类 号：Q943.2[生物学—植物学]