HPTLC联用像素与光密度法快速定量茶饮料中的EGCG  

HPTLC Combines Pixel and Optical Density Method to Fast Quantitative EGCG in Tea Drinks

作　　者：张延杰 胡志高 席兴军[2] 卫晓 刘霭莎 李汴生[4] 陈益胜 Zhang Yanjie;Hu Zhigao;Xi Xingjun;Wei Xiao;Liu Aisha;Li Biansheng;Chen Yisheng(Tsui Xiangyuan Health Food(Zhongshan)Co.,Ltd.,Zhongshan 528414,Guangdong;China Institute of Standardization,Beijing 100191;School of Food Science,Jiangnan University,Wuxi 214122,Jiangsu;School of Food Science and Engineering,South China University of Technology,Guangzhou 510641;College of Food Science and Engineering,Shanxi Agricultural University,Taigu 030801,Shanxi)

机构地区：[1]咀香园健康食品(中山)有限公司,广东中山528414 [2]中国标准化研究院,北京100191 [3]江南大学食品学院,江苏无锡214122 [4]华南理工大学食品科学与工程学院,广州510641 [5]山西农业大学食品科学与工程学院,山西太谷030801

出　　处：《中国食品学报》2023年第9期306-314,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(21804058)。

摘　　要：以HPTLC分析平台,分别用像素分析法和光密度法,建立并对比两种快速检测茶饮料中EGCG含量的方法。以3种市售茶饮料为代表性样品,对比两种方法的应用性能,评估像素分析替代仪器依赖的光密度检测作为茶饮料中EGCG含量检测的新方法的先进性。结果显示,HPTLC-像素分析方法、HPTLC-光密度方法检测的定量限分别为6.8,5 mg/kg,均可以定量茶饮料中EGCG。两种方法目标物在200~1200 ng/斑点浓度范围内线性相关系数相差不大,均在0.999以上。两种方法的回收率均满足要求,HPTLC-像素分析方法测定3种样品加标回收率分别在102.4%~110.8%,102.3%~118.8%,100.3%~113.5%之间,HPTLC-光密度方法测定3种样品加标回收率分别在:103.5%~110.8%,95.3%~111.2%,99.3%~111.2%之间,表明两种方法具有良好的准确性。两种方法的精密度也相差不大,HPTLC-像素分析方法RSD<6.7%,HPTLC-光密度方法RSD<3.2%。结论:HPTLC检测技术中,简单易用的像素分析可以较好地代替昂贵的仪器化光密度扫描,提高方法的性价比和实用性。This study by HPTLC analysis platform,use the pixels in a parallel analysis and optical density method,set up and compared two kinds of rapid detection of EGCG in tea drinks.With three kinds of tea drinks sold as representative samples,compare two methods of application performance,evaluate alternative instrument depend on the optical density of pixel analysis testing as tea drink.The results showed that the detection limits of HPTLC-pixel analysis method and HPTLC-optical density method were 6.8 mg/kg and 5 mg/kg,respectively,which were sufficient to quantify EGCG in tea beverages;Two methods of target in 200-1200 ng/band concentration within the scope of the linear correlation coefficient were small and reached more than 0.999.The recoveries of the two methods meet the requirements,the spiked recoveries of the three samples determinedby the HPTLC-pixel analytical method ranged from 102.4%to 110.8%,102.3%to 118.8%and 100.3%to 113.5%,the spiked recoveries of the three samples determinedby the HPTLC-optical density method ranged from 103.5%to 110.8%,95.3%to 111.2%,and 99.3%to 111.2%,respectively.Indicating that the two methods have good accuracy.The precision of the two methods is also similar,HPTLC-pixel analytical method RSD<6.7%,HPTLC-optical density method RSD<3.2%.In conclusion,in HPTLC detection technology,simple and easy-to-use pixel analysis can better replace expensive instrumented densitometric scanning,improving the cost-effectiveness and practicability of the method.

关 键 词：HPTLC 像素分析 光密度检测 茶饮料 

分 类 号：TS275.2[轻工技术与工程—农产品加工及贮藏工程] O657.72[轻工技术与工程—食品科学与工程]