Fluoxetine Ameliorates the Aggravation of UC Symptoms in C57BL/6 Mice Induced by CUMS  

作　　者：Teng ZHAO Yu-xin ZHOU Rong-jie WANG Ping WAN Ying LI Liang-liang ZHOU Zhen-hua CHEN 

机构地区：[1]Jiangxi Province Key Laboratory of Drug Design and Evaluation,School of Pharmacy,Jiangxi Science and Technology Normal University,Nanchang 330013,China [2]Department of Liver Disease,the Ninth Hospital of Nanchang,Nanchang 330006,China

出　　处：《Current Medical Science》2023年第5期1033-1042,共10页当代医学科学（英文）

基　　金：supported by grants from the National Natural Science Foundation Youth Project of China(No.81703716);Jiangxi Science Foundation for Distinguished Young Scholars(No.20224ACB216019);the Natural Science Foundation of Jiangxi Province(No.20202BABL206151 and No.20202BABL216026);Youth Talents Project of Jiangxi Science and Technology Normal University(No.2017QNBJRC006);Doctoral Startup Fund of Jiangxi Science and Technology Normal University(No.2019BSQD015);Department Education Science and Technology Research Project of Jiangxi(No.GJJ201134);the Open Project of Jiangxi Provincial Key Laboratory of Drug Design and Evaluation(No.JKD-KF-2104);the National Undergraduate Training Program for Innovation of China(No.202211318024).

摘　　要：Objective Patients with chronic ulcerative colitis(UC)often have mental symptoms such as depression and anxiety,and stress can lead to gastrointestinal diseases.However,the correlation between mental stress and UC is unclear.In this paper,chronic unpredictable mild stress(CUMS)was utilized to evaluate the involvement of mental factors in the pathogenesis of UC.Methods The CUMS model was used to evaluate the direct/indirect involvement of mental factors in the pathogenesis of UC.The behavior was evaluated by the open field,forced swimming,and tail suspension tests.Body weight,the disease activity index(DAI)score,colon length,and HE staining of colon tissue were used to evaluate the action of CUMS and fluoxetine.Results The results showed that weight loss and the DAI score increased in CUMS mice,but they had no meaningful effect on colon length and morphological structure of colon tissue.However,CUMS aggravated dextran sulfate sodium(DSS)-induced colon length shortening and colon morphological structure damage.Fluoxetine significantly improved the DAI score,shortened colon length,and damaged morphology and structure of the colons induced by CUMS combined with DSS in mice.Fluoxetine also decreased the level of IL-6 in the serum and the TNF-αand IFN-γlevels of colon tissue.Fluoxetine simultaneously improved behavioral abnormalities induced by CUMS combined with DSS in mice.Conclusion CUMS aggravated the UC symptoms induced by DSS,and fluoxetine could improve the UC symptoms due to its improvement in the inflammatory level and behavioral abnormalities.

关 键 词：ulcerative colitis chronic unpredictable mild stress FLUOXETINE dextran sulfate sodium BEHAVIOR 

分 类 号：R575[医药卫生—消化系统]