Urinary analysis based on surface-enhanced Raman scattering for the noninvasive screening of lung cancer  

作　　者：Xueqian Ren Liping Huang Chengde Wang Yuancai Ge Kaili Zhang Danfeng Jiang Xiaohu Liu Qingwen Zhang Yi Wang 

机构地区：[1]School of Biomedical Engineering,School of Ophthalmology and Optometry,Eye Hospital,Wenzhou Medical University,Wenzhou 325001,China [2]Engineering Research Center of Clinical Functional Materials and Diagnosis&Treatment Devices of Zhejiang Province,Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou 325001,China [3]The First Affiliated Hospital,Wenzhou Medical University,Wenzhou 325001,China [4]School of Opto-Electronic Engineering,Changchun University of Science and Technology,Changchun 130022,China

出　　处：《Engineered Regeneration》2022年第4期387-396,共10页再生工程（英文）

基　　金：supported by Zhejiang Province Natural Science Fund for Distinguished Young Scholars(LR19H180001);Pub-lic Projects of Wenzhou(G2020005);the Startup Fund of Wenzhou Institute,UCAS(WIUCASQD2021031).

摘　　要：We developed a reliable simple analysis of adenosine,creatinine and other nucleosides(cytidine and guanosine)with single measurement based on surface-enhanced Raman spectroscopy(SERS)using DSP-AuNPs with Cu^(2+)ions probe and applied it to achieve noninvasive screening of lung cancer.The gold nanoparticles(AuNPs)were modified with 3,3′-dithiodipropionic acid di(N-hydroxysuccinimide ester)(DSP),which could react with above mentioned molecules followed with the aggregation of AuNPs at the presence of Cu^(2+),thus form the plasmonic hot spots to dramatically increase their fingerprint Raman signal.The probe of DSP-AuNPs was applied for the detail analysis of urinary adenosine of healthy people and lung cancer patients.The SERS measurement indicated a higher concentration of urinary adenosine for lung cancer patients than that of healthy people,which was consistent with the results measured by high-performance liquid chromatography(HPLC).In combination of Raman spectroscopy with the orthogonal partial least squares discriminant analysis(OPLS-DA)model,we are able to discriminate the lung cancer patients from healthy people with the urinary test.The results indicated that besides of adenosine,other metabolites including uric acid,guanine and creatinine may also be the potential tumor markers in urine for the noninvasive lung cancer diagnosis.Such a method paves a way for the noninvasive cancer screening and it can be further modified for the detection of other molecules on medical diagnosis.

关 键 词：ADENOSINE Surface-enhanced Raman scattering Gold nanoparticles OPLS-DA Lung cancer Noninvasive diagnosis 

分 类 号：R734.2[医药卫生—肿瘤]