Liver spheroids on chips as emerging platforms for drug screening  

作　　者：Danqing Huang Xiaoxuan Zhang Xiao Fu Yan Zu Weijian Sun Yuanjin Zhao 

机构地区：[1]Department of Rheumatology and Immunology,Institute of Translational Medicine,The Affiliated Drum Tower Hospital of Nanjing University Medical School,Nanjing 210002,China [2]State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096,China [3]Department of Gastrointestinal Surgery,The Second Affiliated Hospital of Wenzhou Medical University,Wenzhou 325027,China [4]Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine,Vision and Brain Health),Wenzhou,Zhejiang 325001,China [5]Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou,Zhejiang 325001,China

出　　处：《Engineered Regeneration》2021年第1期246-256,共11页再生工程（英文）

基　　金：supported by the National Key Research and Development Program of China(2020YFA0908200);the National Natural Science Foundation of China(52073060,and 61927805).

摘　　要：Liver plays a critical role in drug metabolism and nowadays multi-cellular culture systems aiming at imitating liver-specific morphology and functionality are advancing robustly.Numerous immortalized or stem cell-induced hepatic cell lines have been investigated to enhance the hepatic phenotype in establishing co-culture systems.Owing to the robust progresses of microtechnology and bioengineering,two-dimensional(2D)co-cultures such as sandwich culture and micropatterned co-culture systems have emerged.Controllably arranging the hepatocytes and fibroblasts allows bio-mimic homotypic and heterotypic interactions,and the miniaturized co-culture platform realizes high-throughput and sensitive drug screening.Yet,to address the rapid dedifferentiation and the decreased maintenance of hepatic functions existing in 2D cellular systems,various three-dimensional(3D)and dynamic hepatocyte co-culturing formats have been established to obtain more physiologically relevant liver microsystems and prolonged hepatic functionalities,such as spheroid barcodes,liver organoids,bioengineered hepatic spheroids,and microfluidic perfused liver-on-a-chip.In this review,we first introduce the typical structural color spheroid barcodes which facilitate multiple screening and testing.Then we sketch various advances in liver-imitating cellular co-culture protocols through representative examples.Meanwhile,we summarize current difficulties and propose the prospects of bioengineered liver-on-barcode systems for reliable and high-throughput drug screening.

关 键 词：BARCODE Hepatocyte 3d co-culture SPHEROID Liver-on-a-chip 

分 类 号：R57[医药卫生—消化系统]