铅对砷的毒性效应及毒代动力学过程的影响  

The influence of lead on toxic effect of arsenic and its toxicokinetic process

作　　者：陈鑫 左俊丽 栾宁 崔文文 张惠贤[1] 姚晶晶[1] 严伟[1] CHEN Xin;ZUO Junli;LUAN Ning;CUI Wenwen;ZHANG Huixian;YAO Jingjing;YAN Wei(Hubei Key Laboratory of Nutritional Quality and Safety of Agro-Products,Institute of Quality Standard and Testing Technology for Agro-Products,Hubei Academy of Agricultural Sciences,Wuhan,430064,China;College of Fisheries,Huazhong Agricultural University,Wuhan,430070,China)

机构地区：[1]湖北省农业科学院农业质量标准与检测技术研究所农产品营养品质与安全湖北省重点实验室,武汉430064 [2]华中农业大学水产学院,武汉430070

出　　处：《环境化学》2023年第10期3351-3358,共8页Environmental Chemistry

基　　金：湖北省重点研发计划项目(2021BBA227);湖北省自然科学基金(2021CFB063)资助.

摘　　要：为探究铅对砷的毒性效应的影响及其原因,本研究选用斑马鱼作为模式生物开展了毒理实验,以评价砷和铅的复合毒性效应,分析斑马鱼体内重金属残留量,并采用一级一室动力学模型模拟了砷的毒代动力学过程.毒理实验结果表明,铅对斑马鱼未产生明显致死效应,砷对斑马鱼的致死效应随暴露时间增长显著增强,而铅的共存能够显著降低砷对斑马鱼的致死性,砷和铅对斑马鱼的致死性呈现出拮抗效应.毒代动力学结果表明,铅的存在显著加快了斑马鱼对砷的消除速率并减弱了吸收速率,可降低斑马鱼对砷的富集能力,从而削弱了砷对斑马鱼的毒性效应.本研究揭示了砷和铅对斑马鱼的拮抗效应并从污染物毒代动力学的角度解释了复合毒性形成原因,充实了重金属的复合毒性效应基础数据,可提高重金属风险评估的准确性.To explore the influence of lead on arsenic toxicity and its formation mechanism,zebrafish was selected as model organism to conduct toxicology test in this study.Joint toxicity of arsenic and lead was evaluated.Body residue of metals in zebrafish was analyzed and toxicokinetic process of arsenic was simulated by first order one compartment toxicokinetic model.The toxicology test results indicated lead did not generate lethal effect for zebrafish,but lethal effect caused by arsenic increased significantly with the increase of exposure time.However,the coexistence of lead can significantly reduce the lethality of arsenic to zebrafish.Arsenic and lead showed antagonistic effects on zebrafish lethality.The toxicokinetic results showed the presence of lead significantly accelerated the elimination rate and reduced the uptake rate of arsenic in zebrafish.Thus,the bioconcentration ability of arsenic in zebrafish was weakened and the toxic effect of arsenic on zebrafish was mitigated.This study revealed the antagonistic effect of arsenic and lead on zebrafish and explained the mechanism of joint toxicity from the perspective of toxicokinetic.It provides basic data of joint toxicity for metals and improved the accuracy of risk assessment of metals.

关 键 词：砷 铅 毒性效应 毒代动力学 斑马鱼 

分 类 号：X171.5[环境科学与工程—环境科学]