基于非靶向代谢组学研究普洱熟茶对D-半乳糖致衰老小鼠的抗衰老作用  

Non-targeted Metabolomic Study on Anti-aging Effect of Ripe Pu-erh Tea on D-Galactose-Induced Aging Mice

作　　者：雷舒雯 张智芳[1] 谢桂华 山波 苗玥 赵春燕 陈德洪 侯艳[1,2] 龚加顺[1,4] LEI Shuwen;ZHANG Zhifang;XIE Guihua;SHAN Bo;MIAO Yue;ZHAO Chunyan;CHEN Dehong;HOU Yan;GONG Jiashun(College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;College of Tea,Yunnan Agricultural University,Kunming 650201,China;College of Veterinary Medicine,Yunnan Agricultural University,Kunming 650201,China;Institute of Agro-products Processing,Yunnan Academy of Agricultural Sciences,Kunming 650233,China)

机构地区：[1]云南农业大学食品科学技术学院,云南昆明650201 [2]云南农业大学茶学院,云南昆明650201 [3]云南农业大学动物医学院,云南昆明650201 [4]云南省农业科学院农产品加工研究所,云南昆明650233

出　　处：《食品科学》2023年第15期137-146,共10页Food Science

基　　金：云南省重大科技专项计划项目(202102AE090027);云岭学者专项(YNWR-YLXZ-2018-026);国家自然科学基金地区科学基金项目(81860608);云南省农业基础研究联合专项(2017FG001-011)。

摘　　要：延缓衰老已成为当今社会关注和探究的热点。本课题组前期研究发现普洱熟茶能够通过调节肠道菌群来延缓小鼠衰老,但小鼠体内内源性物质反应生成的代谢产物不明。本研究采用Morris水迷宫实验检测对照小鼠、D-半乳糖衰老模型小鼠及普洱熟茶干预小鼠的学习记忆能力,并利用非靶向代谢组学技术检测各组小鼠的脑组织和血清,探究普洱熟茶对D-半乳糖致衰老小鼠的抗衰老作用,筛选普洱熟茶对衰老小鼠作用的差异代谢物并分析其涉及的代谢通路。结果表明:普洱熟茶能改善衰老小鼠的学习行为能力,调节衰老小鼠脑组织中26种差异代谢物,主要涉及甘油磷脂代谢、VB_(6)代谢、组氨酸代谢和嘌呤代谢通路,其中甘油磷脂代谢和组氨酸代谢通路最为显著;血清中共有11种差异代谢物,主要涉及VB_(6)代谢和花生四烯酸代谢通路,其中VB_(6)代谢通路最为显著。给予普洱熟茶的衰老小鼠体内甘油磷脂代谢通路相关代谢物磷脂酰胆碱(phosphatidylcholine,PC)(20:5/20:4)、磷脂酰乙醇胺(phosphatidyl ethanlamine,PE)(22:2/14:0)、磷脂酰丝氨酸(phosphatidylserine,PS)(20:5/18:1)、溶血磷脂酰胆碱(lysophosphatidylcholine,Lyso PC)(18:2)等,组氨酸代谢通路相关代谢物肌肽,VB_(6)代谢通路相关代谢物5’-磷酸吡哆醛丰度显著升高,表明普洱熟茶可能通过调节脂类代谢、氨基酸代谢发挥抗衰老作用。Delaying aging has become a hot spot of social concern and research.Our previous studies have shown that ripe Pu-erh tea can delay aging in mice by regulating the intestinal flora,but the metabolites in response to endogenous substances in mice are not clear.In this paper,the Morris water maze test was used to detect learning and memory capacity in control,D-galactose-induced aging,and ripe Pu-erh tea-treated mice.Non-targeted metabolomics was used to detect metabolites in the brain tissue and serum of mice from each group for the purpose of exploring the anti-aging effect of ripe Pu-erh tea on D-galactose-induced aging mice,screening differential metabolites among the three groups and analyzing the related metabolic pathways.The results showed that ripe Pu-erh tea improved learning capacity,and regulated 26 differential metabolites in the brain tissue of aging mice,mainly involved in the glycerophospholipid metabolism,vitamin B_(6)metabolism,histidine metabolism and purine metabolism pathways,among which the glycerophospholipid metabolism and histidine metabolism pathway were the most significant.A total of 11 differential metabolites were identified in serum,mainly involved in the metabolism of vitamin B_(6)and arachidonic acid,among which vitamin B_(6)metab olism pathway was the most significant.After the intervention with ripe Pu-erh tea,the contents of glycerophospholipid metabolites including phosphatidylcholine[PC(20:5/20:4)],phosphatidyl ethanlamine[PE(22:2/14:0)],phosphatidylserine[PS(20:5/18:1)]and lysophosphatidylcholine[LysoPC(18:2)],the histidine metabolite carnosine,and the vitamin B_(6)metabolite pyridoxal 5’-phosphate were significantly increased in aging mice.These results suggest that ripe Pu-erh tea can delay aging by regulating lipid and amino acid metabolism.

关 键 词：普洱熟茶 D-半乳糖 脑组织 血清 非靶向代谢组学 衰老 

分 类 号：TS201.4[轻工技术与工程—食品科学]