β-1,3-葡聚糖酶的结构、功能及应用研究进展  

Research Progress on Structure,Function and Application ofβ-1,3-Glucanases

作　　者：魏夏森 余赛男 张哲一 高海燕[1] 秦臻 WEI Xiasen;YU Sainan;ZHANG Zheyi;GAO Haiyan;QIN Zhen(School of Life Sciences,Shanghai University,Shanghai 200444,China)

机构地区：[1]上海大学生命科学学院,上海200444

出　　处：《食品科学》2023年第15期269-277,共9页Food Science

基　　金：国家自然科学基金面上项目(32172160,31972120)。

摘　　要：β-1,3-葡聚糖酶是一类能够特异性水解β-1,3-葡聚糖中β-1,3-糖苷键的酶类,其水解生成的产物为一系列不同大小的寡糖或单糖。β-1,3-葡聚糖酶在食品功能性寡糖制备、果蔬保鲜、生物医药、植物抗病等领域具有重要应用价值。已发现的β-1,3-葡聚糖酶主要归属于12个糖苷水解酶(glycoside hydrolases,GH)家族,包括GH16、GH17、GH55、GH64、GH81、GH128和GH132等。β-1,3-葡聚糖酶广泛存在于细菌、真菌、植物和昆虫中,因来源和序列进化关系的差异,其结构和催化功能也呈现多样性。酶的结构与功能是探索酶催化反应机理、挖掘酶催化特性以及酶分子改造研究的基础。因此,本文从β-1,3-葡聚糖酶的结构、功能及应用等方面,对国内外β-1,3-葡聚糖酶的研究现状进行综述,以期为β-1,3-葡聚糖酶相关基础研究及应用开发提供参考。β-1,3-Glucanases are enzymes that specifically hydrolyzeβ-1,3-glycosidic linkages bonds inβ-1,3-glucan to generate a range of oligosaccharides or monosaccharides.β-1,3-Glucanases have important potential applications in functional oligosaccharide preparation,fruit and vegetable preservation,biopharmaceuticals,and plant disease resistance.β-1,3-Glucanases belonging to 12 glycoside hydrolase(GH)families have been identified,including GH16,GH17,GH55,GH64,GH81,GH128 and GH132.β-1,3-Glucanases are widely distributed in bacteria,fungi,plants and insects,which exhibit diverse structures and catalytic functions due to differences in sources and sequence evolution.Structural and functional studies of enzymes are the basis for exploring the catalytic mechanism,enzyme properties,and molecular modification.Therefore,this paper reviews the current state of research on the structure,function and application ofβ-1,3-glucanases,in order to provide a reference for the basic research and application ofβ-1,3-glucanases.

关 键 词：Β-1 3-葡聚糖酶 糖苷水解酶 结构与功能 催化机制 应用 

分 类 号：Q814.4[生物学—生物工程]