壳聚糖基碳点纸芯片检测槐米中芦丁含量  

Detection of Rutin in Flower Buds of Sophora japonica by Using Chitosan-Based Carbon Dot Paper Chip

作　　者：乐薇 蒙利秀 王源 覃永薇 YUE Wei;MENG Lixiu;WANG Yuan;QIN Yongwei(College of Environmental and Biological Engineering,Wuhan Technology and Buniness Universtity,Wuhan 430065,China)

机构地区：[1]武汉工商学院环境与生物工程学院,湖北武汉430065

出　　处：《食品科学》2023年第16期260-266,共7页Food Science

基　　金：湖北省自然科学基金项目(2019CFC920);湖北省优势特色学科群“资源环境与智能化工程”项目;武汉工商学院学术团队项目(XSTD2015003)。

摘　　要：基于黄酮类物质芦丁对天冬酰胺热解法制备的荧光碳点有选择性的猝灭作用,研究一种壳聚糖基碳点纸芯片快速检测槐米中芦丁含量的方法。滤纸浸没于含碳点与壳聚糖的铸膜液中,干燥后滤纸表面形成碳点壳聚糖薄膜,获得壳聚糖基碳点纸芯片,直接点样后,样品斑点可限制在一定区域内且显色均匀,在365 nm紫外灯照射下拍照,分析显色斑点的红(R)、绿(G)、蓝(B)值,优化纸芯片的制备条件和检测条件。结果表明优化条件为:铸膜液组成为壳聚糖质量浓度20 mg/mL、碳点质量浓度1.0 mg/mL、样品溶剂为50%乙醇溶液、显色时间20 min,该优化条件下芦丁质量浓度(C)在4~120μg/mL范围时,样品斑点与溶剂斑点的G值差值(ΔG)和C呈线性关系。按该法测得3批次槐米中芦丁相对含量平均值分别为23.85%、22.83%、20.30%,平均相对标准偏差分别为6.1%、5.6%、6.7%,回收率为91.27%~107.5%,与高效液相法测定结果接近。该法简便快速,可用于槐米芦丁含量测定的快速现场测定。Based on the selective quenching effect of the flavonoid rutin on the fluorescence of carbon dots prepared by asparagine pyrolysis,a method for rapid detection of rutin content in the flower buds of Sophora japonica by using chitosan-based carbon dot paper chip was developed.A filter paper was immersed in the casting solution of carbon dots and chitosan.After drying,a carbon dot chitosan film was formed on the surface of the filter paper,so that the chitosan-based carbon dot paper chip was obtained.After samples were dripped on the chip,chromogenic reaction occurred uniformly within a limited region,and pictures were taken under an ultraviolet(UV)lamp at 365 nm for analysis of the red(R),green(G),and blue(B)values of the colored spots in order to optimize the preparation conditions and detection conditions.The results showed that the optimal conditions were as follows:chitosan concentration of 20 mg/mL,carbon spot concentration of 1.0 mg/mL,50%ethanol as sample solvent,and reaction time of 20 min.Under these conditions,the difference(ΔG)in G-value between sample and solvent spots had a linear relationship with rutin concentration(C)in the range of 4 to 120 mg/mL.Using this method,the average contents of rutin in the flower buds of S.japonica from three batches were determined to be 23.85%,22.83%and 20.30%,the average relative standard deviations(RSDs)were 6.1%,5.6%and 6.7%,and the recoveries were 91.27%to 107.5%,which were close to the results of high performance liquid chromatography(HPLC).The proposed method is simple and rapid,and can be used for rapid on-site determination of rutin content in the flower buds of S.japonica.

关 键 词：碳点 壳聚糖 纸芯片 芦丁 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.3[轻工技术与工程—食品科学与工程]