Protective effect of lycopene on Parkinson's disease cell model based on endoplasmic reticulum stress  

作　　者：BAO Bo CHAI Xing-xing DENG Zi-liang LIU Lu-lu ZHU Shao-ping LI Li-li 

机构地区：[1]Department of Pathology and Pathophysiology,Guangdong Medical University,Zhanjiang 524023,China [2]Laboratory Animal Center,Guangdong Medical University,Dongguan 523808,China [3]Department of Histology and Embryology,Guangdong Medical University,Dongguan 523808,China [4]Dongguan Key Laboratory for Development and Application of Experimental Animal Resources in Biomedical Industry,Dongguan 523808,China

出　　处：《Journal of Hainan Medical University》2023年第14期15-21,共7页海南医学院学报（英文版）

基　　金：Science and Technology Program of Guangdong Province(No.2013B060300038);Scientific Research Foundation of Guangdong Medical University(No.GDMUM2019029)。

摘　　要：Objective:To evaluate the effect of lycopene on Parkinson's disease cell model and its possible mechanism.Methods:The SH-SY5Y cells were treated with 0.5μmol/L rotenone for 24 h to establish Parkinson's disease cell model.The experiments were randomly divided into the control group,the lycopene group,the rotenone group,the pretreatment groups of different concentrations lycopene(low,medium,high concentration).Cell viability was detected by CCK-8 assay,the morphological changes of cells were observed under an inverted microscope,Hoechst staining was used to observe cell apoptosis,the expression and distribution of endoplasmic reticulum stress marker proteins GRP78 and CHOP in each group were detected by Western blot and cell immunofluorescence.Results:The study found that compared with the control group,the cell viability in the rotenone group was significantly decreased with obvious apoptosis;compared with the rotenone group,the cell viability of the lycopene pretreatment group was improved,and the difference was statistically significant(P<0.05);The apoptosis in the lycopene pretreatment group was decreased.The expression of GRP78 and CHOP in the rotenone group was significantly higher than that in the control group(P<0.01),while the expression of both in the high concentration lycopene pretreatment group was lower than that in the rotenone group(P<0.05).Conclusion:Lycopene pretreatment had a significant protective effect on rotenone-induced SH-SY5Y cells,which may be related to the fact that lycopene pretreatment can effectively alleviate endoplasmic reticulum stress in SH-SY5Y cells damaged by rotenone.

关 键 词：LYCOPENE ROTENONE Parkinson's disease Endoplasmic reticulum stress 

分 类 号：R742.5[医药卫生—神经病学与精神病学]