草果果实的化学成分及其对α-葡萄糖苷酶的抑制活性研究  

Study on the chemical constituents of the fruits of Amomum tsao-ko and their α -glucosidase inhibitory activity

作　　者：吕福杰 路继刚 范在举 邓爱霞[2] LYU Fu-jie;LU Ji-gang;FAN Zai-ju;DENG Ai-xia(Liaocheng Traditional Chinese Medicine Hospital;School of Materials Science and Engineering,Liaocheng University,Liaocheng 252000,China)

机构地区：[1]聊城市中医医院 [2]聊城大学材料科学与工程学院,聊城252000

出　　处：《天然产物研究与开发》2023年第11期1887-1893,共7页Natural Product Research and Development

基　　金：山东省自然科学基金(ZR2021ME126)。

摘　　要：本研究通过系统分离探索了中药草果果实的化学成分。采用多种柱色谱手段对其乙醇提取物进行分离纯化,通过波谱法鉴定化合物的结构并通过PNPG法测定化合物对α-葡萄糖苷酶抑制活性。从中药草果果实的乙醇提取物乙酸乙酯萃取部位分离鉴定出11个化合物,分别为(R)-1-(1-ethoxypropyl)-3,5-dimethoxyphenol(1)、(R)-1-(3,4,5-trimethoxyphenyl)propan-1-ol(2)、3-甲氧基-4-羟基苯丙酮(3)、香草乙酮(4)、2,6二甲氧基-4甲基苯酚(5)、香兰素(6)、4-萜烯醇(7)、methyl(9 S,10 R,11 E,13 R,15 Z)-9,10,13-trihydroxyoctadeca-11,15-dienoate(8)、methyl(9 S,10 R,11 E,13 R)-9,10,13-trihydroxyoctadec-11-enoate(9)、amomutsaoko A(10)以及renealtin A(11)。其中化合物1为酚类化合物,是作为天然产物首次报道,化合物10和11具有强于阿卡波糖的α-葡萄糖苷酶抑制活性。This study explored the chemical components of fruits of the Chinese medicine Amomum tsao-ko through systematic separation.Various column chromatography techniques were used to separate and purify the ethanol extract and the structure of the compounds was identified mainly by spectral analysis.The inhibitory activity of the compounds onα-glucosidase was also studied by a PNPG method.The 11 compounds isolated from the ethyl acetate extract of the ethanol extract of the fruits of Amomum tsao-ko were named(R)-1-(1-ethoxypropyl)-3,5-dimethoxyphenol(1),(R)-1-(3,4,5-trimethoxyphenyl)propan-1-ol(2),4-hydroxy-3-methoxypropiophenone(3),apocynin(4),4-methyl-2,6-dimethoxyphenol(5),4-hydroxy-3-methoxybenzaldehyde(6),1-terpinen-4-ol(7),methyl(9 S,10 R,11 E,13 R,15 Z)-9,10,13-trihydroxyoctadeca-11,15-dienoate(8),methyl(9 S,10 R,11 E,13 R)-9,10,13-trihydroxyoctadec-11-enoate(9),amomutsaoko A(10)and renealtin A(11).Compound 1 is a phenolic compound and first reported as a natural product,compounds 10 and 11 have stronger inhibitory activity againstα-glucosidase than acarbose.

关 键 词：草果 豆蔻属 酚类化合物 Α-葡萄糖苷酶 

分 类 号：R932[医药卫生—生药学]