基于HS-SPME-GC-MS测定5种蔷薇属植物花瓣挥发性成分  

Detection of Volatile Organic Compounds in Five Rose Petals Based on Headspace Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry

作　　者：王洪乾 寇亚平 姚晨阳 孟昭阳 王晶晶 贾瑞冬[1] 赵鑫[1] 葛红[1] 杨树华[1] WANG Hongqian;KOU Yaping;YAO Chenyang;Meng Zhaoyang;WANG Jingjing;JIA Ruidong;ZHAO Xin;GE Hong;YANG Shuhua(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences/State Key Laboratory of Vegetable Biobreeding/Key Laboratory of Biology and Genetic Improvement of Flower Crops(North China),Ministry of Agriculture and Rural Affairs,Beijing 100081)

机构地区：[1]中国农业科学院蔬菜花卉研究所/蔬菜生物育种全国重点实验室/农业农村部花卉生物学与种质创制重点实验室(北方),北京100081

出　　处：《植物遗传资源学报》2023年第6期1626-1638,共13页Journal of Plant Genetic Resources

基　　金：山东省重点研发计划项目(2022LGZCQY009);现代农业产业技术体系北京市创新团队(BAIC09-2023);中央级公益性科研院所基本科研业务费专项(IVF-BRF2021016,IVF-BRF2022014);国家重点研发计划项目(2018YFD1000401)。

摘　　要：花瓣中挥发性成分对观赏植物的开发和利用起着重要作用。本研究采用顶空固相微萃取气质色谱联用技术,对5种蔷薇盛花期花瓣中挥发性成分进行检测分析,结果表明:5种蔷薇植物花瓣中共鉴定出91种挥发性成分,包含萜烯类、醇类、酯类、醛类、烷类、酚类、酮类、醚类和酸类9个类型。其中,百叶蔷薇共检测到45种挥发性成分,无特有成分,百叶蔷薇品种摩洛哥共检测到52种挥发性成分,5种特有成分;格拉斯共检测到40种挥发性成分,2种特有成分;菲茨詹姆斯共检测到57种挥发性成分,8种特有成分;大马士革蔷薇共检测出63种挥发性成分,11种特有成分。2-苯乙醇、香叶醇、香茅醇、乙酸香叶酯等是5种蔷薇花瓣中主要的挥发性成分,大马士革蔷薇花瓣的挥发性物质含量最多,达3622.68 ng/g,其次是摩洛哥、菲茨詹姆斯、格拉斯和百叶蔷薇,挥发性物质含量分别为2507.0 ng/g、2445.9 ng/g、1394.54 ng/g和883.38 ng/g。基于5种蔷薇花瓣中挥发性成分含量进行聚类分析发现,大马士革蔷薇与菲茨詹姆斯聚类关系较近,萜烯类挥发性成分种类和含量在5种蔷薇中差异较大。本研究为蔷薇属植物资源的开发和利用提供理论参考。Fragrance volatile organic compounds play key role on exploitation and utilization in ornamental plants.In this study,headspace solid phase microextraction combined with gas chromatography mass spectrometry(HS-SPME-GC-MS)was used to determine and quantify the volatile organic compounds(VOCs)in five rose petals.Ninety-one VOCs were identified and classified into nine types:terpenes,alcohols,esters,aldehydes,alkanes,phenols,ketones,ethers,acids.Forty-five components were detected in R.centifolia;52 components including five exclusive compositions were detected in R.‘Morocco';40 compounds including two exclusive compositions were detected in R.‘de Grasse';57 compounds including eight exclusive compositions were detected in R.‘Duc de Fitzjames';63 compounds including 1l exclusive compositions were detected in R.×damascena.The compounds 2-Phenylethanol,Geraniol,β-Citronellol,and Geranyl acetate were predominant in five rose petals.R.×damascena had the highest content of VOCs,reaching 3622.68 ng/g,and the contents of VOCs in R.‘Morocco',R.‘Duc de Fitzjames',R.‘de Grasse',R.centifolia were 2507.07 ng/g,2445.9 ng/g,1394.54 ng/g,883.38 ng/g,respectively.Heatmap cluster analysis and cluster analysis showed that R.×damascena was closely related to R.Duc de Fitzjames',and the content of terpenes were significantly different in petals of five rose.Collectively,this study provided reference for future exploitation and utilization of Rosa genus resources.

关 键 词：蔷薇属 挥发性成分 HS-SPME-GC-MS 主成分分析 

分 类 号：S685.12[农业科学—观赏园艺]