植物乳植杆菌YZH81胞外多糖基因簇的功能鉴定  

Identification of A Novel Capsular Polysaccharide Cluster in Lactiplantibacillus plantarum YZH81

作　　者：赵芳[1] 黄璐 宦海琳[3] 黄淑婷 张文君[1] 梁晴晴 杨瑶[1] ZHAO Fang;HUANG Lu;HUAN Hailin;HUANG Shuting;ZHANG Wenjun;LIANG Qingqing;YANG Yao(School of Food Science and Pharmaceutical Engineering,Nanjing Normal University,Nanjing 210023,China;School of Tourism&Culinary Arts,Zhejiang Business College,Hangzhou 310053,China;Institute of Animal Science,Jiangsu Academy of Agricultural Science,Nangjing 210014,China;Xuzhou Xiangran Agriculture and Animal Husbandry Development Co.,Ltd.,Xuzhou 221622,China)

机构地区：[1]南京师范大学食品与制药工程学院,江苏南京210023 [2]浙江商业职业技术学院旅游烹饪学院,浙江杭州310053 [3]江苏省农业科学院畜牧研究所,江苏南京210014 [4]徐州湘然农牧发展有限公司,江苏徐州221622

出　　处：《食品工业科技》2023年第23期118-126,共9页Science and Technology of Food Industry

基　　金：浙江商业职业技术学院人才启动项目3010203025(KT21001232);江苏省农业科技自主创新资金项目(CX[22]2019);江苏省苏北科技专项(XZ-SZ202113)。

摘　　要：为了研究植物乳植杆菌胞外多糖(EPS)的合成机制,本文选取一株具有较高EPS产量的植物乳植杆菌YZH81开展其EPS合成基因簇(cps)的研究。经全基因组测序、比对和分析,结果确定了YZH81菌株基因组含有两个cps基因簇,其中一个cps基因簇(cps1)的结构与功能尚未鉴定,而另一个cps基因簇(cps2)与其他已报道的植物乳植杆菌同源性很高。本研究实验敲除了cps1基因簇,与野生型YZH81菌株相比,YZH81△cps1菌株的EPS产量降低了52.28%,自聚集能力加速,粘附性降低,且清除1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picryl-hydrazyl radical,DPPH·)能力下降了32.42%。该结果说明YZH81菌株的cps1基因簇与EPS合成相关,也为进一步研究该菌株EPS生物合成机制建立有利条件。In order to investigate the mechanism of extracellular polysaccharide(EPS)synthesis in Lactobacillus plantarum,a strain of Lactobacillus plantarum YZH81 with high EPS production was selected for the study of extracellular polysaccharide synthesis gene cluster(cps).After whole-genome sequencing,alignment and analysis,it was determined that the genome of YZH81 contained two cps gene clusters,one of which(cps1)had yet to be identified in terms of structure and function,while the other(cps2)was highly homologous to other reported Lactobacillus plantarum strains.The conclusions in this study,the cps1 gene cluster resulted 52.28%reduction in EPS production,accelerated self-aggregation,reduced adhesion and 32.42%reduction in the ability to generated 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH·)in the YZH81Δcps1 strain compared to the wild-type YZH81 strain.The results suggested that the cps1 gene cluster of strain YZH81 was associated with EPS synthesis and established favorable conditions for further studies on the mechanism of EPS biosynthesis in this strain.

关 键 词：植物乳植杆菌 胞外多糖(EPS) 荚膜多糖簇(cps cluster) 粘附性 益生菌 

分 类 号：Q933[生物学—微生物学]