高产抗菌脂肽Fengycin芽孢杆菌的诱变育种和发酵条件优化  

Mutation Breeding and Optimization of Fermentation Conditions of Bacillus Highly Producing Antimicrobial Lipopeptide Fengycin

作　　者：陈尚里 于福田 沈圆圆 刘小玲[1] CHEN Shangli;YU Futian;SHEN Yuanyuan;LIU Xiaoling(College of Light Industry and Food Engineering,Guangxi University,Nanning 530004,China)

机构地区：[1]广西大学轻工与食品工程学院,广西南宁530004

出　　处：《食品工业科技》2023年第23期134-143,共10页Science and Technology of Food Industry

基　　金：国家重点研发计划(2018YFD0901003);广西科技重大专项(桂科AA20302019-7)。

摘　　要：为了提高Fengycin产量,以芽孢杆菌YA-215为出发菌,通过复合诱变(紫外诱变、ARTP-LiCl诱变)育种来获取高产Fengycin突变体。通过单因素实验和响应面试验等确定最佳发酵工艺优化。结果表明,复合诱变选育获得一株高产Fengycin突变株UA397,全基因组测序结合16S进化样本分析显示为暹罗芽孢杆菌。其最佳发酵工艺条件为:蔗糖25 g/L、蛋白胨30 g/L、发酵温度37.7℃、发酵时间37.8 h、接种量5.01%。在此发酵条件下,暹罗芽孢杆菌UA-397的Fengycin产量为517.09 mg/L,是野生型在未进行发酵条件优化时Fengycin产量113.02 mg/L的4.575倍。研究结果为抗菌脂肽Fengycin应用于食品、医药和生物防治等领域奠定了产量基础。In order to increase the yield of Fengycin,Bacillus YA-215 was used as the starting strain in this study to obtain high-yield Fengycin mutants through compound mutagenesis(UV mutagenesis,ARTP-LiCl mutagenesis)breeding.The optimum fermentation conditions were determined by single-factor experiment and response surface methodology.The results showed that a high-yielding Fengycin mutant strain UA397 was obtained through compound mutagenesis,and the whole genome sequencing combined with 16S evolution sample analysis showed that it was Bacillus siamensis.The optimum fermentation process conditions were:Sucrose 25 g/L,peptone 30 g/L,fermentation temperature 37.7℃,fermentation time 37.8 h,inoculum size 5.01%.Under these conditions,the Fengycin yield of Bacillus siamensis UA-397 was 517.09 mg/L,which was 4.575 times the Fengycin yield of 113.02 mg/L of the wild type without optimizing the fermentation conditions.This study laid the yield basis for the application of antibacterial lipopeptide Fengycin in the fields of food,medicine and biological control.

关 键 词：暹罗芽孢杆菌 FENGYCIN 诱变育种 响应面 发酵优化 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]