基于碳氮稳定同位素比值和UPLC-Q-Orbitrap代谢组学技术的有机奶和普通奶的成分差异研究  

Differentiated Study of Constituents between Organic Milk and Ordinary Milk by Stable Isotope Ratio and UPLC-Q-Orbitrap

作　　者：聂雪梅[1,2] 谢昀 许博舟 许秀丽 NIE Xuemei;XIE Yun;XU Bozhou;XU Xiuli(Institute of Food Safety,Chinese Academy of Inspection&Quarantine,Beijing 100176,China;Key Laboratory of Food Quality and Safety for State Market Regulation,Beijing 100176,China)

机构地区：[1]中国检验检疫科学研究院食品安全研究所,北京100176 [2]国家市场监管重点实验室(食品质量与安全),北京100176

出　　处：《食品工业科技》2023年第23期246-252,共7页Science and Technology of Food Industry

基　　金：中国检科院基本科研业务费项目(2020JK008)。

摘　　要：目的:为了防止受利益驱动的一些不法商家随意标注“有机”字样来对食品采取假冒、伪造、以次充好等方式欺诈消费者。方法:本研究提出采用碳氮稳定同位素比值和UPLC-Q-Orbitrap代谢组学技术两种方式对有机奶和普通奶进行差异成分的研究,同时对不同泌乳期的有机奶进行分析。结果:由于碳和氮同位素比值单因素方差分析P<0.01,因此有机奶和普通奶具有显著性差异,可通过碳和氮同位素比值对有机奶进行判别;同时基于液相色谱-高分辨质谱的差异物质的代谢组学数据,采用OPLS-DA方法筛选出差异代谢物,共筛选包括组氨酸等5种差异较大的代谢物,且不同泌乳期的有机奶中组氨酸等化合物含量差异性较大。结论:该研究虽然样本有限,但有机奶和普通奶存在显著的差异代谢物,通过C和N同位素比值,以及5种筛查的差异代谢物可初步判定有机奶,该研究对有机奶和普通奶的差异化研究提供一定的科学依据。Objective:To prevent some illegal companies driven by interests from arbitrarily labeling"organic"to deceive consumers using ways such as counterfeiting,forgery,and shoddy food.Methods:This study investigated differential components between organic and regular milk by stable isotope ratio and UPLC-Q-Orbitrap-based metabolomics.Organic milk with different lactation stages was also analyzed.Results:There was a significant difference between organic and ordinary milk for single factor ANOVA P<0.01.The carbon stable isotope and nitrogen stable isotope ratios could be used to distinguish organic and ordinary milk.Based on the data from UPLC-Q-Orbitrap,differential metabolites were screened by the OPLS-DA method,five different constituents were obtained,and there was a big difference between different lactation stages of organic milk such as histidine etc.Conclusion:Although the data of this study was obtained from limited samples,the different constituents between organic and ordinary milk were confirmed.Organic milk could be preliminarily determined by the data of carbon isotope ratio,nitrogen isotope ratio,and five different constituents screened by the UPLC-Q-Orbitrap method.This study could provide a scientific basis for the identification of organic and ordinary milk.

关 键 词：有机奶 碳氮同位素比值 UPLC-Q-Orbitrap 成分差异 

分 类 号：TS207.7[轻工技术与工程—食品科学]