基于比色试纸的人工食品色素可视化即时检验  

Colorimetric paper-based visual point-of-care tests of artificial food colorants

作　　者：王海丽 李凤 刘淑雯 王双寿 WANG Haili;LI Feng;LIU Shuwen;WANG Shuangshou(School of Chemistry and Chemical Engineering,Anhui University of Technology,Maanshan,Anhui 243032,China;Hudong Road No.2 Primary School,Maanshan,Anhui 243000,China)

机构地区：[1]安徽工业大学化学与化工学院,安徽马鞍山243032 [2]马鞍市湖东路第二小学,安徽马鞍山243000

出　　处：《贵州师范大学学报（自然科学版）》2023年第6期77-84,100,共9页Journal of Guizhou Normal University：Natural Sciences

基　　金：国家自然科学基金项目(21904003);安徽省高校研究计划项目(2022AH050296);安徽省生化药物工程技术研究中心开放课题(2022SYKFZ02)。

摘　　要：提出一种基于试纸的比色方法用于人工食品色素(Artificial food colorants, AFCs)可视化即时检验。通过脲基硅烷偶联剂在玻纤滤纸表面的缩聚一步制得试纸,萃取AFCs后即得颜色梯度与AFCs浓度梯度对应的比色卡,据此实现AFCs半定量比色分析。结果表明,所得试纸具有极佳的可着色性,AFCs着色浓度低至10-6M,且整个比色测定过程在0.5 min内完成。最终该试纸被成功地用于实际饮料样品中AFCs的可视化检测,测定结果与仪器分析技术高度一致。An indicator paper-based colorimetric assay was proposed for visual point-of-care tests(POCTs)of AFCs.The indicator papers were prepared in one-step by the polycondensation of ureido group-contained silane coupling agents onto glass microfiber filter paper,and the colorimetric cards with fine color mappings to AFCs concentration gradients were obtained via AFCs extraction by indicator papers,and the semiquantitative colorimetry was consequently achieved.The experimental findings showed that the presented indicator papers were of superb colorability with a colorable concentration of AFCs as low as the level of 10-6 M,enabling to fast end the whole POCTs process within 0.5 min.Finally,the indicator papers were successfully applied to the visual detection of AFCs in real beverage samples,and the results were highly consistent with those of instrumental techniques.

关 键 词：人工食品色素 食品安全 比色分析 即时检验 

分 类 号：TS207.3[轻工技术与工程—食品科学]