芹菜APX基因家族鉴定及其表达分析  

Identification and expression analysis of APX gene family in celery(Apium graveolens L.)

作　　者：杜佳庚 周瑾 李晓燕 张颖 伍丽梅 李梦瑶 DU Jiageng;ZHOU Jin;LI Xiaoyan;ZHANG Ying;WU Limei;LI Mengyao(College of Horticulture,Sichuan Agricultural University,Chengdu,Sichuan 611130,China)

机构地区：[1]四川农业大学园艺学院,四川成都611130

出　　处：《干旱地区农业研究》2023年第6期54-63,共10页Agricultural Research in the Arid Areas

基　　金：国家自然科学基金项目(32002027);四川省自然科学基金项目(2022NSFSC1674)。

摘　　要：抗坏血酸过氧化物酶(Ascorbate peroxidase, APX)是植物活性氧代谢中重要的抗氧化酶之一,在植物抵抗氧化胁迫方面发挥重要作用。利用生物信息学方法对芹菜基因组中的APX基因家族成员进行鉴定和分析,并通过实时荧光定量PCR(quantitative real-time PCR, qRT-PCR)验证分析AgAPXs在高温胁迫下的表达情况,为开展芹菜APX基因参与高温胁迫调控机制提供依据。结果表明:芹菜基因组中共有9个APX基因,随机分布在5个染色体上,并出现了基因片段复制现象;大多数基因被定位在细胞质中。系统发育分析表明,AgAPX基因家族可分为3个亚族,同一亚族中的成员具有相似的基因结构和基序。启动子顺式元件分析表明,大多数AgAPX基因含有多种与生长发育、植物激素和逆境胁迫相关的顺式元件。高温胁迫下,芹菜APX活性提高。qRT-PCR分析表明,AgAPXs在不同时间的高温处理下表达具有显著差异,并与转录组表达丰度相一致,AgAPX2、AgAPX3、AgAPX4、AgAPX5、AgAPX7的表达量和APX活性具有显著相关性,推测AgAPXs可能参与了芹菜抵御高温的调控过程。本研究初步鉴定并提供了芹菜APX基因家族成员信息,为今后进一步探索芹菜APX基因功能提供了重要的研究基础。Ascorbate peroxidase(APX)is one of the important antioxidant enzymes in plant reactive oxygen species metabolism and plays an important role in plant resistance to oxidative stress.In this study,bioinformatics was used to identify and analyze APX gene family members in celery genome,and the expression of AgAPXs under high temperature stress was verified and analyzed by quantitative real-time PCR(qRT-PCR),which provided a basis for participation of celery APX gene in the regulation mechanism of high temperature stress.Results showed that a total of 9 APX genes were systematically identified from celery,which were randomly distributed on 5 chromosomes,and gene fragment duplication occurred.Most genes were located in the cytoplasm.Phylogenetic analysis showed that the AgAPX gene family could be divided into three subtypes,and members of each subtype shared similar gene structures and motifs.Promoter cis element analysis showed that most AgAPX genes contain a variety of cis elements related to growth and development,plant hormones and stress.Under high temperature stress,the APX activity of celery increased.qRT-PCR analysis showed that AgAPXs were differentially expressed under high temperature treatment at different times,were consistent with the transcriptome expression abundance.The expression of AgAPX2,AgAPX3,AgAPX4,AgAPX5 and AgAPX7 was significantly correlated with the activity of APX,which could be speculated that this gene was involved in the regulation of celery tolerance to high temperature.The results preliminarily identified and provided information on the members of celery APX gene family,which provided an important research basis for further research on the function of celery APX gene in the future.

关 键 词：芹菜 APX 系统进化 顺式元件 高温胁迫应答 

分 类 号：S636.3[农业科学—蔬菜学] Q945.78[农业科学—园艺学]