RNA sequencing of exosomes secreted by fibroblast and Schwann cells elucidates mechanisms underlying peripheral nerve regeneration  

作　　者：Xinyang Zhou Yehua Lv Huimin Xie Yan Li Chang Liu Mengru Zheng Ronghua Wu Songlin Zhou Xiaosong Gu Jingjing Li Daguo Mi 

机构地区：[1]Suzhou Medical College of Soochow University,Suzhou,Jiangsu Province,China [2]Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education,Co-Innovation Center of Neuroregeneration,Nantong University,Nantong,Jiangsu Province,China [3]Department of Orthopedic,Nantong Traditional Chinese Medicine Hospital,Nantong,Jiangsu Province,China [4]Nantong Stomatological Hospital Affiliated to Nantong University,Nantong,Jiangsu Province,China [5]Department of General Practice,Affiliated Hospital of Nantong University,Nantong,Jiangsu Province,China

出　　处：《Neural Regeneration Research》2024年第8期1812-1821,共10页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.81870975(to SZ)。

摘　　要：Exosomes exhibit complex biological functions and mediate a variety of biological processes,such as promoting axonal regeneration and functional recove ry after injury.Long non-coding RNAs(IncRNAs)have been reported to play a crucial role in axonal regeneration.Howeve r,the role of the IncRNA-microRNAmessenger RNA(mRNA)-competitive endogenous RNA(ceRNA)network in exosome-mediated axonal regeneration remains unclear.In this study,we performed RNA transcriptome sequencing analysis to assess mRNA expression patterns in exosomes produced by cultured fibroblasts(FC-EXOs)and Schwann cells(SCEXOs).Diffe rential gene expression analysis,Gene Ontology analysis,Kyoto Encyclopedia of Genes and Genomes analysis,and protein-protein intera ction network analysis were used to explo re the functions and related pathways of RNAs isolated from FC-EXOs and SC-EXOs.We found that the ribosome-related central gene Rps5 was enriched in FC-EXOs and SC-EXOs,which suggests that it may promote axonal regeneration.In addition,using the miRWalk and Starbase prediction databases,we constructed a regulatory network of ceRNAs targeting Rps5,including 27 microRNAs and five IncRNAs.The ceRNA regulatory network,which included Ftx and Miat,revealed that exsosome-derived Rps5 inhibits scar formation and promotes axonal regeneration and functional recovery after nerve injury.Our findings suggest that exosomes derived from fibro blast and Schwann cells could be used to treat injuries of peripheral nervous system.

关 键 词：ceRNA network EXOSOMES fibroblast cells Gene Ontology(GO) Kyoto Encyclopedia of Genes and Genomes(KEGG) protein-protein interaction(PPI)networks RNA-seq Schwann cells 

分 类 号：R741[医药卫生—神经病学与精神病学]