免疫磁珠高通量自动净化-超高效液相色谱法快速测定饲料中玉米赤霉烯酮  

Immunomagnetic Bead High-Throughput Automatic Purification-Ultra-High Performance Liquid Chromatography for Determination of Zearalenone in Feed

作　　者：王蒙[1,2] 陈金男 叶金 李丽 王松雪 张颖 吴宇[2] 刘洪美 Wang Meng;Chen Jinnan;Ye Jin;Li Li;Wang Songxue;Zhang Ying;Wu Yu;Liu Hongmei(School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093;Institute of Grain and Oil Quality Safety,Academy of National Food and Strategic Reserves Administration,Beijing 102629)

机构地区：[1]上海理工大学健康科学与工程学院,上海200093 [2]国家粮食和物资储备局科学研究院粮油质量安全研究所,北京102629

出　　处：《中国粮油学报》2023年第9期29-34,共6页Journal of the Chinese Cereals and Oils Association

基　　金：国家重点研发计划项目(2019YFC1605301);广西重点研发计划项目(桂科AB20238018);中国科协青年人才托举工程项目(2021QNRC001)。

摘　　要：采用免疫磁珠净化饲料原料及其产品中的玉米赤霉烯酮,实现玉米赤霉烯酮的自动富集、清洗和洗脱,结合超高效液相色谱对玉米赤霉烯酮进行分析测试。本文对饲料样品稀释液进行了优化。根据3倍信噪比的峰响应值,本方法玉米赤霉烯酮的检出限为4.5μg/kg,定量限为15.0μg/kg,在10~500 ng/mL范围内线性关系良好,相关系数(R^(2))为0.99993。玉米赤霉烯酮在鸡饲料和猪饲料样品中的加标回收率为92.6%~112.9%,相对标准偏差为1.0%~8.8%。对同一饲料基质进行连续4 d同一浓度的加标实验,回收率为97.0%~98.9%,日间精密度在5.7%~5.9%。与免疫亲和柱法进行比较,检测了19种饲料样品,t检验显示方法与免疫亲和柱法无显著性差异。免疫磁珠自动净化法可同时净化24个样品,无需人员手动操作,减少实验误差,提高检测效率。Zearalenone in feed materials and products was purified by immunomagnetic beads to realize automatic enrichment,cleaning and elution of zearalenone,and analyzed and tested by UPLC.In this paper,feed sample dilutions were optimized.The detection limit of zearalenone was 4.5 μg/kg,the limit of quantification was 15.0μg/kg,and the linearity was good in the range of 10-500 ng/mL,and the correlation coefficient(R^(2)) was 0.999 93.The recoveries of zearalenone in chicken feed and pig feed were 92.6%-112.9% with relative standard deviations(RSD) of 1.0%-8.8%.The recoveries were 97.0%-98.9% and the daytime precision was 5.7%-5.9%.Compared with immunoaffinity column method,19 kinds of feed samples were detected,and the t-test revealed that,the differences between this method and the immunoaffinity column method were not significant.The automatic immunomagnetic beads purification method could simultaneously purify 24 samples and reduce experimental error without manual operation,improving detection efficiency.

关 键 词：免疫磁珠 自动净化 超高效液相色谱 饲料 玉米赤霉烯酮 

分 类 号：TS207.3[轻工技术与工程—食品科学]