苏姜猪线粒体DNA D-Loop区遗传多样性及系统进化研究  

Genetic diversity and phylogenetic evolution in mitochondrial DNA D-Loop region of Sujiang pigs

作　　者：陶勇[1,2] 任善茂 董晓君[3] 赵旭庭 TAO Yong;REN Shanmao;DONG Xiaojun;ZHAO Xuting(Jiangsu Agri-animal Husbandry Vocational College,Taizhou 225300,China;Jiangsu Sujiang Pig Breeding Co.,Ltd.,Taixing 225400,China;Animal Health Supervision Institute of Huaian District,Huaian City,Jiangsu Province,Huaian 223001,China)

机构地区：[1]江苏农牧科技职业学院,江苏泰州225300 [2]江苏苏姜种猪有限公司,江苏泰兴225400 [3]江苏省淮安市淮安区动物卫生监督所,江苏淮安223001

出　　处：《黑龙江畜牧兽医》2023年第22期51-56,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金：江苏现代农业产业技术体系建设项目(JATS[2021]387);江苏农牧科技职业学院科技项目(NSF2021TC04)。

摘　　要：为了研究苏姜猪的遗传多样性和系统地位,试验采集47头苏姜猪的耳组织进行基因组DNA提取,利用PCR技术扩增线粒体DNA(mitochondrial DNA,mtDNA)D-Loop区序列并测序,对47条苏姜猪mtDNA D-Loop区序列的碱基组成和含量、序列长度、变异位点、单倍型数量及单倍型多样性和核苷酸多态性指标进行了分析,估计序列变异程度,并构建系统发育树和分析单倍型网络关系,最后利用其他24个猪种的mtDNA D-Loop区序列构建了苏姜猪mtDNA D-Loop区序列系统发育树。结果表明:苏姜猪mtDNA D-Loop区全长序列为1194~1274 bp,A、T、C、G碱基含量分别为32.9%、24.8%、26.5%、15.8%;47条序列共鉴定出3种单倍型和8个变异位点(均为简约性信息位点);单倍型多样度为0.00173,核苷酸多样度为0.499,平均核酸差异数为1.210,Tajima’s D值为-0.91674(P>0.10),符合中性突变;该苏姜猪群有3个分支,推测有3个母系起源,并与长江流域以南猪种有较近的亲缘关系。说明苏姜猪群体遗传多样性较为丰富,具有3个母系来源。In order to study the genetic diversity and systematic status of Sujiang pigs,the ear tissues of 47 Sujiang pigs were collected for genomic DNA extraction,and the complete mtDNA D-Loop region sequence was amplified by PCR and sequenced.The composition and content,sequence length,variation sites,haplotype number,haplotype diversity and nucleotide polymorphism indexes of 47 Sujiang-pig mtDNA D-Loop region sequences were analyzed to estimate the degree of sequence variation.The phylogenetic tree was constructed and the haplotype network relationship was analyzed.Finally,the haplotype phylogenetic tree of mtDNA D-Loop region of Sujiang pig was constructed using the mtDNA D-loop sequence of other 24 pig breeds.The results showed that the full-length sequence of Sujiang pig mtDNA D-Loop region was 1194-1274 bp,and the content of A,T,C,and G base were 32.9%,24.8%,26.5%and 15.8%,respectively.A total of three haplotypes and eight variation sites(all reduced information loci)were identified from 47 sequences.Haplotype diversity was 0.00173;the nucleotide diversity was 0.499;the mean nucleic acid differences was 1.210,and the Tajima's D value was-0.91674(P>0.10),which was consistent with neutral mutations.The Sujiang pig herd had three branches,and it was speculated that it had three maternal origins,and it was closely related to the pig breeds in the south region of the Yangtze River.These results indicated that the genetic diversity of Sujiang pig population was relatively rich,with three maternal origins.

关 键 词：苏姜猪 mtDNA D-Loop区 序列分析 单倍型 遗传多样性 系统进化 

分 类 号：S828.8[农业科学—畜牧学]