木榄内生真菌Penicillium sp.GXIMD00006的次级代谢产物及其降血糖活性研究  

Secondary Metabolites and Its Hypoglycemic of Endophytic Fungus Penicillium sp.GXIMD00006 Derived from the Bruguiera gymnorrhiza

作　　者：肖泽恩 邹思华 江娇 罗海梅 黄锡山 刘永宏 谭振 XIAO Ze’en;ZOU Sihua;JIANG Jiao;LUO Haimei;HUANG Xishan;LIU Yonghong;TAN Zhen(Institute of Marine Drugs,Guangxi University of Chinese Medicine,Nanning Guangxi 530200,China;Guangxi Key Laboratory of Marine Drugs(Guangxi University of Chinese Medicine),Nanning Guangxi 530200,China;School of Chemistry and Pharmacy,Guangxi Normal University,Guilin Guangxi 541004,China)

机构地区：[1]广西中医药大学海洋药物研究院,广西南宁530200 [2]广西海洋药物重点实验室(广西中医药大学),广西南宁530200 [3]广西师范大学化学与药学学院,广西桂林541004

出　　处：《广西师范大学学报（自然科学版）》2023年第6期150-157,共8页Journal of Guangxi Normal University:Natural Science Edition

基　　金：广西科技基地和人才专项(桂科AD19245123);广西中医药大学面上项目(2022MS037);广西中医药大学大学生科研训练课题(2021DXS11)。

摘　　要：为挖掘木榄内生真菌的降血糖功效,对木榄内生真菌Penicillium sp.GXIMD00006进行扩大培养,采用柱层析技术对其次级代谢产物进行分离纯化,利用NMR、MS等技术结合文献对比对分得的化合物进行鉴定。结果表明:共获得11个化合物,分别为:(+)-sclerotiorin(1)、sclerotioramine(2)、isochromophiloneⅧ(3)、terretonin(4)、terretonin A(5)、chrodrimanin A(6)、chrodrimanin B(7)、chrodrimanin E(8)、3,5-二羟基麦角甾醇-7,22-二烯-6-酮(9)、麦角甾醇(10)和过氧化麦角甾醇(11),其中,2个萜类衍生物4和5均为首次从青霉属中分离得到。该研究首次发现化合物1具有较强的α-葡萄糖苷酶抑制活性,IC_(50)值为3.30μmol/L,表明木榄内生真菌的次级代谢产物具有开发成为新的α-葡萄糖苷酶抑制剂的潜力。In order to explore the hypoglycemic effect of endophytic fungi from the mangrove Bruguiera gymnorrhiza,the endophytic fungus Penicillium sp.GXIMD00006 was fermented and isolated by various column chromatography techniques.By using MS,NMR and literature comparison,11 compounds were identified as(+)-sclerotiorin(1),sclerotioramine(2),isochromophilone VIII(3),terretonin(4),terretonin A(5),chrodrimanin A(6),chrodrimanin B(7),chrodrimanin E(8),(22 E,24 R)-3β,5α-dihydroxy-ergosta-7,22-dien-6-one(9),erogosterol(10)and ergosterol peroxide(11).Besides,terretonin(4)and terretonin A(5)were first isolated from the Penicillium sp.Theα-glucosidase inhibitory effects showed that(+)-sclerotiorin(1)had significant inhibitory activity with IC_(50)of 3.30μmol/L,which indicated that the secondary metabolites of endophytic fungi from the Bruguiera gymnorrhiza had the potential to be developed into newα-glucosidase inhibitors.

关 键 词：内生真菌 次级代谢产物 木榄 阿扎菲酮 Α-葡萄糖苷酶 抑制剂 降血糖 

分 类 号：R284[医药卫生—中药学]