直立型半野生大豆叶绿体基因组分析  

Chloroplast Genome Analysis of An Erect Semi-Wild Soybean

作　　者：郭冉昊 赵淑文 米福贵[1] 候伟峰 赵力兴 GUO Ran-hao;ZHAO SHU-wen;MI FU-gui;HOU WEI-feng;ZHAO LI-xing(College of Grassland,Resources and Environment,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010000,China;National Experimental Teaching Demonstration Center of Botany,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010000,China;Hinggan League Institute of Agricultural and Animal Husbandry Sciences,Hinggan League,Inner Mongolia 137400,China)

机构地区：[1]内蒙古农业大学草原与资源环境学院,内蒙古呼和浩特010000 [2]内蒙古农业大学植物学国家级实验教学示范中心,内蒙古呼和浩特010000 [3]兴安盟农牧科学研究所,内蒙古兴安盟137400

出　　处：《草地学报》2023年第11期3334-3342,共9页Acta Agrestia Sinica

基　　金：兴安盟野生植物资源调查研究资助。

摘　　要：采用Illumina高通量测序技术对直立型半野生大豆(Glycine gracilis)叶绿体基因组(Chloroplast DNA,cpDNA)进行测序和组装,获得完整基因组,以探究其结构特征,为后续基于cpDNA的相关分析以及大豆资源多样性保护和系统发育等研究提供基础或依据。结果表明,直立型半野生大豆cpDNA全长148320 bp,为典型四分体结构,注释基因108个,包含66个蛋白编码基因、29个tRNA基因和4个rRNA基因。基因组序列上共检测到87个SSR位点,其中与单核苷酸、双核苷酸、三核苷酸、四核苷酸、五核苷酸对应的位点分别为55,19,4,7,2个。在系统发育上,直立型半野生大豆与已公布的普通型一年生半野生大豆(Glycine gracilis)、栽培大豆(Glycine max)及野大豆(Glycine soja)亲缘关系较近。Illumina high-throughput sequencing technology was used to sequence and assemble the chloroplast genome of an erect semi-wild soybean(Glycine gracilis)to obtain a complete chloroplast genome,so as to explore its structural characteristics,and provide a basis for subsequent related analysis on chloroplast genome and diversity protection and phylogeny of soybean resources.The results demonstrated that the full-length chloroplast genome of that erect semi-wild soybean was 148320 bp,which was a typical tetrad structure.108 genes were annotated,including 66 protein-coding genes,29 tRNA genes and 4 rRNA genes.A total of 87 SSR loci were detected in the genome sequence,of which 55,19,4,7 and 2 loci were corresponding to mononucleotide,dinucleotide,trinucleotide,tetranucleotide and pentanucleotide,respectively.Based upon the phylogeny analysis,that erect semi-wild soybean was closely related to the published common annual semi-wild soybean(Glycine gracilis),cultivated soybean(Glycine max),and wild soybean(Glycine soja).

关 键 词：半野生大豆 CPDNA 简单重复序列 密码子偏好性 系统发育 

分 类 号：S565.1[农业科学—作物学]