灵芝-黄芪药渣固体双向发酵工艺优化及菌质多糖对肠癌HCT116的抑制作用研究  

Optimization of solid bi-directional fermentation process of Ganoderma lucidum-Astragalus residue and mycoplasmic polysaccharide antitumor activity to HCT116 cancer

作　　者：杨英歌[1] 李荣 宋凯[1] 王娟[1] 王丹[1] YANG Yingge;LI Rong;SONG Kai;WANG Juan;WANG Dan(Xuzhou Vocational College of Bioengineering,Xuzhou 221006;Shandong City Service Institute,Yantai 264670)

机构地区：[1]徐州生物工程职业技术学院,徐州221006 [2]山东城市服务职业学院,烟台264670

出　　处：《中国食品添加剂》2023年第11期110-118,共9页China Food Additives

基　　金：徐州市科技项目(KC22014)。

摘　　要：为了高值化利用黄芪药渣,通过单因素和响应面优化灵芝-黄芪药渣固体双向发酵生产多糖的工艺,最佳发酵工艺为料液比0.66 g/mL、装瓶量0.22 g/mL、接种量4.5%、发酵温度28℃、发酵时间16 d,菌质多糖平均产率达到(29.21±0.23)mg/g。HPLC分析菌质多糖的单糖组成,菌质多糖由甘露糖、鼠李糖、葡萄糖、半乳糖和阿拉伯糖组成。采用MTT法测定菌质多糖对人体肠癌HCT116细胞的体外抗肿瘤活性,当菌质多糖浓度超过40 mg/mL时,对人体肠癌HCT116细胞的体外抑制率超过85%。菌质多糖溶液经口灌胃,测定对移植于裸鼠的人体肠癌HCT116的抑制作用,剂量为25 mL/kg时,对移植于裸鼠的人体肠癌HCT116的抑瘤率为55.99%。In order to utilize astragalus residue,the solid bi-directional fermentation process of Ganoderma lucidum-astragalus residue to produce polysaccharides was studied by single factor test and response surface method.The optimal fermentation technology were as follows:material-liquid ratio of 0.66 g/mL,medium-flask ratio of 0.22 g/mL,inoculum size of 4.5%,fermentation temperature of 28℃,and fermentation time 16 d.The average yield of polysaccharides reached 29.21±0.23 mg/g.The monosaccharides were analyzed by HPLC.Mycoplasmic polysaccharide consists of mannose,rhamnose,glucose,galactose and arabinose.The antitumor activity of mycoplasma polysaccharide on HCT116 cells in vitro was determined by MTT assay.When the concentration of mycoplasmic polysaccharide was more than 40 mg/mL,the inhibition rate of HCT116 cells in vitro was more than 85%.The inhibitory effect of mycoplasma polysaccharide solution on HCT116 cells transplanted into nude mice was determined by oral gavage.The inhibitory rate on HCT116 was 55.99%when mycoplasma polysaccharide dose was at 25 mL/kg.

关 键 词：灵芝 黄芪药渣 菌质多糖 抗肿瘤活性 

分 类 号：O629.12[理学—有机化学] TS205[理学—化学]