基于叶绿体基因组数据的芸香科属间系统发育初步分析  

Preliminary Analysis of Intergeneric Phylogenetic of Rutaceae Based on Chloroplast Genomes Data

作　　者：刘涵 孙冲 吴杰 黄威[1] 黄勤琴 刘霞 LIU Han;SUN Chong;WU Jie;HUANG Wei;HUANG Qinqin;LIU Xia(College of Landscape Architecture and Life Science,Chongqing University of Arts and Sciences,Chongqing 402160,China;College of Biology and Food Engineering,Chongqing Three Gorges University,Chongqing 404000,China;College of Horticulture and Landscape Architecture,Yangtze University,Jingzhou,Hubei 434025,China;College of Horticulture and Landscape Architecture,Southwest University,Chongqing 402160,China)

机构地区：[1]重庆文理学院园林与生命科学学院,重庆402160 [2]重庆三峡学院生物与食品工程学院,重庆404000 [3]长江大学园艺与园林学院,湖北荆州434025 [4]西南大学园艺与园林学院,重庆400799

出　　处：《西北植物学报》2023年第11期1866-1871,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金项目(31901324);重庆市博士后研究特别资助项目(2021XM2010);重庆市教委青年项目(KJQN202201337,KJQN202101320,KJQN201801344)。

摘　　要：为探究花椒属种水平系统进化关系以及为新品种培育研究奠定理论基础,该研究以芸香科物种的叶片为材料,采用改良CTAB法提取叶绿体DNA,利用BGISEQ-500平台进行叶绿体基因组测序,并对叶绿体基因组进行组装、注释,联合NCBI数据库数据,共获得芸香科19属49个物种的全叶绿体基因组序列。构建了芸香科属间系统进化关系。结果表明,(1)基于叶绿体基因组序列矩阵(总长196641 bp),ML和BI 2种方法得到的系统发育树的拓扑结构基本一致,系统发育树各分支具有较强的支持率,叶绿体基因组数据可以解决芸香科属间的系统发育关系。(2)芸香科为单系类群,并进一步形成两大分支,其中柑橘亚科为单系,与芸香亚科内的芸香属聚为分支I,分支Ⅱ由芸香亚科和飞龙掌血亚科组成,两亚科均不是单系,其中飞龙掌血亚科的香肉果属,茵芋属与芸香亚科的白鲜属,臭常山属是最早分化出来的类群,其次是蜜茱萸属和山油柑属;黄檗属和吴茱萸属与花椒属类群形成姊妹群,飞龙掌血属的物种飞龙掌血嵌套于花椒属分支中,支持飞龙掌血物种并入花椒属的处理。To investigate the phylogenetic relationship of Zanthoxylum species level and provide a theoretical foundation for the cultivation of new varieties,in this study,we used the leaves of Rutaceae species as materials to extract chloroplast DNA using modified CTAB method,sequenced the chloroplast genome by BGISEQ-500 platform,assembled and annotated the chloroplast genome.Combined with NCBI database data,we obtained the whole chloroplast genome sequences of 49 species in 19 genera of Rutaceae.The results showed that,(1)based on the chloroplast genome sequence matrix(total length was 196641 bp),the topological nodes of phylogenetic trees obtained by ML and BI methods were basically the same,with strong support for each branch.The chloroplast genome data could resolve the phylogenetic relationships of intergeneric in Rutaceae.(2)Rutaceae was a monophyletic group and further forms two branches,of which subfamily Aurantioideae was a monophyletic group and clustered with Ruta in Rutaceae into Clade I.CladeⅡwas composed of Rutoideae and Toddalioideae,neither of which was monophyletic group.Among them,Casimiroa and Skimmia of Toddalioideae and Dictamnus and Orixa of Rutoideae were the early-diverging clade,followed by Melicope and Acronychia.Phellodendron and Tetradium formed a clade sister to Zanthoxylum.Toddalia asiatica was nested in Zanthoxylum,and the result supported the treatment of merging T.asiatica into Zanthoxylum.

关 键 词：系统发育 叶绿体基因组 系统进化 花椒属 芸香科 

分 类 号：Q789[生物学—分子生物学] S722[农业科学—林木遗传育种]