补料策略优化促进乳酸乳球菌HB03发酵合成Nisin  

Optimization of Fed-batch Culture Strategy for Improved Synthesis of Nisin by Lactococcus lactis HB03

作　　者：熊华仪 陈曦[1] 刘月锋 陈雄[1] 李沛[2] 王志[1] XIONG Huayi;CHEN Xi;LIU Yuefeng;CHEN Xiong;LI Pei;WANG Zhi(Key Laboratory of Fermentation Engineering(Ministry of Education),Hubei Collaborative Innovation Center of Industrial Fermentation,Hubei University of Technology,Wuhan 430068,China;Hubei Key Laboratory of Yeast Function,Angela Yeast Co.Ltd.,Yichang 443003,China)

机构地区：[1]湖北工业大学发酵工程教育部重点实验室,工业发酵省部共建协同创新中心,湖北武汉430068 [2]安琪酵母股份有限公司,酵母功能湖北省重点实验室,湖北宜昌443003

出　　处：《食品科学》2023年第22期218-224,共7页Food Science

基　　金：湖北省区域创新发展科技专项(2020BGC010);工业发酵省部共建协同创新中心项目(2022KF25)。

摘　　要：为提高乳酸乳球菌HB03发酵合成Nisin的效率,本研究基于乳酸乳球菌发酵生长期间存在的氧化胁迫效应和胞外氨基酸消长规律,分析了肽水解酶系、UMP从头合成和肽聚糖合成代谢在转录组水平的表达差异,确定对数中后期Nisin合成限制因素为半胱氨酸(cysteine,Cys)和精氨酸(arginine,Arg)供应不足,并通过氨基酸单因素和碳氮源补加优化,确定10 L发酵罐水平优化的补料策略为:11、13.5、17、19.5 h分别加入0.15 mmol/L的Cys,12 h补入蛋白胨(15 g/L),10~24 h补入蔗糖(1.5 g/(L·h)),12~21 h流加Arg(0.25 g/(L·h))。在此条件下Nisin效价达到了8963 IU/mL,比只补加Cys发酵效价(6993 IU/mL)提高了28.2%,研究结果可为Nisin工业发酵提供重要参考。To improve the efficiency of nisin synthesis by Lactococcus lactis HB03,the oxidative stress effect and the pattern of changes in the concentration of extracellular amino acids during the growth and fermentation of this strain were determined.Then,the differences in gene expression related to the peptide hydrolase system,UMP de novo synthesis,and peptidoglycan anabolism at the transcriptome level were analyzed.As a result,deficiencies of cysteine(Cys)and arginine(Arg)were identified as the limiting factors for nisin synthesis at the mid to late logarithmic phase.The optimal fed-batch culture strategy in a 10 L fermentor was obtained as follows:addition of 0.15 mmol/L Cys at 11,13.5,17,and 19.5 h,addition of 15 g/L peptone at 12 h,addition of sucrose at a flow rate of 1.5 g/(L·h)during 10 to 24 h,and addition of Arg at a flow rate of 0.25 g/(L·h)during 12 to 21 h.Under these conditions,nisin production was increased to 8963 IU/mL,which was 28.2%higher than that obtained by adding only Cys(6993 IU/mL).These results provide an important reference for the industrial fermentation of nisin.

关 键 词：乳酸乳球菌 乳酸链球菌素 补料批培养优化 发酵合成 

分 类 号：TS209[轻工技术与工程—食品科学]