基于UPLC-MS技术分析沙棘果肉成熟过程中生物活性成分差异  

Differences in Bioactive Composition of Sea Buckthorn Berry Pulp during the Ripening Process as Analyzed by Metabolomics Based on Ultra-high Performance Liquid Chromatography Coupled to Mass Spectrometry

作　　者：丁健 阮成江 杨红 关莹[3] 李贺 陈叶 唐克[3] 吴立仁[3] DING Jian;RUAN Chengjiang;YANG Hong;GUAN Ying;LI He;CHEN Ye;TANG Ke;WU Liren(Institute of Plant Resources,Dalian Minzu University,Dalian 116600,China;College of Resources and Environment,Tibet Agricultural and Animal Husbandry University,Linzhi 860000,China;Heilongjiang Academy of Agricultural Sciences,Harbin 150059,China)

机构地区：[1]大连民族大学资源植物研究所,辽宁大连116600 [2]西藏农牧学院资源与环境学院,西藏林芝860000 [3]黑龙江省农业科学院,黑龙江哈尔滨150059

出　　处：《食品科学》2023年第22期276-286,共11页Food Science

基　　金：国家自然科学基金青年科学基金项目(31800574);国家自然科学基金面上项目(32071799);国家自然科学基金国际(地区)合作与交流项目(32111540255);西藏中央引导地方项目(XZ202201YD0025C);辽宁省自然科学基金项目(2020-MZLH-03;2020-MZLH-09);水利部沙棘开发管理中心项目(2022-zg-kj-018);中央高校基本科研业务费专项(0919-140114)。

摘　　要：为了解沙棘果肉成熟过程中重要生物活性成分的合成代谢差异,采用超高效液相色谱-串联质谱技术,对2个沙棘品系发育期间(绿色和橙色)的果肉进行非靶向代谢组学研究。结果表明:4组样品存在较大的代谢差异,依据不同品系发育期样品组间变量投影重要度>1且t检验的P<0.05,共筛选到124个显著差异代谢物,主要包括有机酸、碳水化合物、脂类、黄酮类和次生代谢物等;根据京都基因与基因组百科全书代谢通路分析,分别识别到15、7个和4个显著差异代谢物与脂类、黄酮和5-羟色胺合成代谢相关,以及其他8个与抗癌功能相关的显著差异代谢物。沙棘果肉磷脂酰胆碱(16:0/16:0)和甘油磷酰胆碱的显著上调与油脂高积累密切相关;显著上调的异鼠李素则是果肉富含黄酮的关键;N-乙酰羟色氨、卡培他滨和γ-氨基丁酸等抗癌相关生物活性成分在果肉发育期间呈现出相对较高的含量。这为解析沙棘生物活性成分合成代谢机制以及相关功能产品开发提供科学依据。In order to reveal the differences in the biosynthesis and metabolism of the major bioactive composition in sea buckthorn berry pulp during ripening,non-targeted metabolomics based on ultra-high performance liquid chromatography coupled to mass spectrometry(UPLC-MS)was used to analyze the metabolites of sea buckthorn berry pulp from two strains at different developmental stages(green and orange pulp).The results showed that the four sample groups had significant metabolic differences.A total of 124 significantly differential metabolites were detected among the four groups based on variable importance in the projection(VIP)>1 and t-test P<0.05,including organic acids,carbohydrates,lipids,flavonoids and secondary metabolites.According to Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis,15,7 and 4 significantly differential metabolites associated with lipid,flavonoid and serotonin metabolism were identified,respectively,and eight significantly differential metabolites involved in anticancer function were selected.The significant up-regulation of phosphatidylcholine(PC)(16:0/16:0)and glycerophosphocholine could be the reason for the high accumulation of lipids in sea buckthorn berry pulp.The significant upregulation of isorhamnetin was key for the high flavonoid content in sea buckthorn berry pulp.Relatively high contents of N-acetylserotonin,capecitabine,γ-aminobutyric acid(GABA)and other bioactive composition involved in anticancer function were shown in sea buckthorn berry pulp during its development.These results provide a scientific basis for revealing the metabolic mechanism of bioactive composition of sea buckthorn berries and developing related functional products.

关 键 词：沙棘 果肉 差异代谢物 油脂 黄酮 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]